Journal article
Metabolic phenotyping using UPLC–MS and rapid microbore UPLC–IM–MS: Determination of the effect of different dietary regimes on the urinary metabolome of the Rat
Chromatographia
2020
Abstract
A rapid reversed-phase gradient method employing a 50 mm × 1 mm i.d., C18 microbore column, combined with ion mobility and high-resolution mass spectrometry, was applied to the metabolic phenotyping of urine samples obtained from rats receiving different diets. This method was directly compared to a “conventional” method employing a 150 × 2.1 mm i.d. column packed with the same C18 bonded phase using the same samples. Multivariate statistical analysis of the resulting data showed similar class discrimination for both microbore and conventional methods, despite the detection of fewer mass/retention time features by the former. Multivariate statistical analysis highlighted a number of ions that represented diet-specific markers in the samples. Several of these were then identified using the combination of mass, ion-mobility-derived collision cross section and retention time including N-acetylglutamate, urocanic acid, and xanthurenic acid. Kynurenic acid was tentatively identified based on mass and ion mobility data.
Details
- Title
- Metabolic phenotyping using UPLC–MS and rapid microbore UPLC–IM–MS: Determination of the effect of different dietary regimes on the urinary metabolome of the Rat
- Authors/Creators
- M. Letertre (Author/Creator)N.C. Munjoma (Author/Creator)S.E. Slade (Author/Creator)R.S. Plumb (Author/Creator)J. Swann (Author/Creator)M. Coen (Author/Creator)J.K. Nicholson (Author/Creator)I.D. Wilson (Author/Creator)
- Publication Details
- Chromatographia
- Publisher
- Springer Nature
- Identifiers
- 991005541158907891
- Copyright
- © 2020 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Harry Butler Institute; Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry