Metabolite Profiling of Annual Ryegrass Cultivars to Assess Disease Resistance and Susceptibility

Pushpendra Koli; Manjree Agarwal; David Kessell; Shalini Mahawar; Xin Du; Yonglin Ren; Simon J. McKirdy

doi:10.1021/acs.jafc.4c03986

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Metabolite Profiling of Annual Ryegrass Cultivars to Assess Disease Resistance and Susceptibility

Journal article

Open access

Peer reviewed

Metabolite Profiling of Annual Ryegrass Cultivars to Assess Disease Resistance and Susceptibility

Pushpendra Koli, Manjree Agarwal, David Kessell, Shalini Mahawar, Xin Du, Yonglin Ren and Simon J. McKirdy

Journal of agricultural and food chemistry, Vol.72(45), pp.24895-25448

2024

DOI: https://doi.org/10.1021/acs.jafc.4c03986

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Abstract

annual ryegrass toxicity

chemometrics

ryegrass cultivars

DI-SPME

GC−MS

forage

metabolites

pattern recognition

principal component analysis

Direct immersion solid-phase microextraction coupled with gas chromatography–mass spectroscopy was used to create chemical fingerprints of annual ryegrass cultivars (Lolium rigidum). Extracts made of the inflorescences of four cultivars and one accession of annual ryegrass were assessed to identify differential metabolites between those resistant to and susceptible to bacterial galls associated with annual ryegrass toxicity (ARGT). Numerous compounds were identified. Principal component analysis showed distinct clustering of metabolites from disease-resistant and disease-susceptible cultivars. Partial least-squares-discriminant analysis identified sterols, esters, aldehydes, and terpenes that correlated with resistance to galls formation. Esters, sterols, phenols, heterocyclics, fatty acids, organofluorides, and siloxanes were predominant in resistant genotypes, whereas alcohols, aldehydes, terpenes, and hydrocarbons were predominant in susceptible genotypes. The identification of differentially expressed metabolites provides potential chemical markers to guide breeding strategies for ARGT resistance in ryegrass.

Details

Title: Metabolite Profiling of Annual Ryegrass Cultivars to Assess Disease Resistance and Susceptibility
Authors/Creators: Pushpendra Koli - Murdoch University
Manjree Agarwal - ChemCentre
David Kessell - Department of Primary Industries and Regional Development
Shalini Mahawar - Murdoch University
Xin Du - Murdoch University
Yonglin Ren - Murdoch University
Simon J. McKirdy - Murdoch University
Publication Details: Journal of agricultural and food chemistry, Vol.72(45), pp.24895-25448
Publisher: ACS Publications
Identifiers: 991005713361807891
Murdoch Affiliation: Murdoch University; Harry Butler Institute; Centre for Biosecurity and One Health
Language: English
Resource Type: Journal article

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