Methodological bias associated with soluble protein recovery from soil

Lucy M Greenfield; Paul W Hill; Eric Paterson; Elizabeth M Baggs; Davey L Jones

doi:10.1038/s41598-018-29559-4

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Methodological bias associated with soluble protein recovery from soil

Journal article

Open access

Peer reviewed

Methodological bias associated with soluble protein recovery from soil

Lucy M Greenfield, Paul W Hill, Eric Paterson, Elizabeth M Baggs and Davey L Jones

Scientific reports, Vol.8, 11186

2018

DOI: https://doi.org/10.1038/s41598-018-29559-4

PMCID: PMC6060134

PMID: 30046143

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Abstract

Proteins play a crucial role in many soil processes, however, standardised methods to extract soluble protein from soil are lacking. The aim of this study was to compare the ability of different extractants to quantify the recovery of soluble proteins from three soil types (Cambisol, Ferralsol and Histosol) with contrasting clay and organic matter contents. Known amounts of plant-derived C-labelled soluble proteins were incubated with soil and then extracted with solutions of contrasting pH, concentration and polarity. Protein recovery proved highly solvent and soil dependent (Histosol > Cambisol > Ferralsol) and no single extractant was capable of complete protein recovery. In comparison to deionised water (10-60% of the total protein recovered), maximal recovery was observed with NaOH (0.1 M; 61-80%) and Na-pyrophosphate (0.05 M, pH 7.0; 45-75% recovery). We conclude that the dependence of protein recovery on both extractant and soil type prevents direct comparison of studies using different recovery methods, particularly if no extraction controls are used. We present recommendations for a standard protein extraction protocol.

Details

Title: Methodological bias associated with soluble protein recovery from soil
Authors/Creators: Lucy M Greenfield - Bangor University
Paul W Hill - Bangor University
Eric Paterson - James Hutton Institute
Elizabeth M Baggs - University of Edinburgh
Davey L Jones - Bangor University
Publication Details: Scientific reports, Vol.8, 11186
Publisher: Springer Nature
Identifiers: 991005560343507891
Copyright: © 2018 The Authors
Murdoch Affiliation: Centre for Sustainable Farming Systems
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.45 Soil Science; 3.45.112 Soil Carbon Dynamics
Web Of Science research areas: Soil Science
ESI research areas: Agricultural Sciences