Journal article
Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy
Clinical and Experimental Pharmacology and Physiology, Vol.44(12), pp.1241-1253
2017
Abstract
We assessed the utility of synchrotron-radiation micro-computed tomography (micro-CT) for quantification of the radial geometry of the renal cortical vasculature. The kidneys of nine rats and six rabbits were perfusion fixed and the renal circulation filled with Microfil. In order to assess shrinkage of Microfil, rat kidneys were imaged at the Australian Synchrotron immediately upon tissue preparation and then post fixed in paraformaldehyde and reimaged 24 hours later. The Microfil shrank only 2-5% over the 24 hour period. All subsequent micro-CT imaging was completed within 24 hours of sample preparation. After micro-CT imaging, the kidneys were processed for histological analysis. In both rat and rabbit kidneys, vascular structures identified in histological sections could be identified in two-dimensional (2D) micro-CT images from the original kidney. Vascular morphology was similar in the two sets of images. Radial geometry quantified by manual analysis of 2D images from micro-CT was consistent with corresponding data generated by light microscopy. However, due to limited spatial resolution when imaging a whole organ using contrast-enhanced micro-CT, only arteries ≥100 and ≥60 μm in diameter, for the rat and rabbit respectively, could be assessed. We conclude that it is feasible and valid to use micro-CT to quantify vascular geometry of the renal cortical circulation in both the rat and rabbit. However, a combination of light microscopic and micro-CT approaches are required to evaluate the spatial relationships between intrarenal arteries and veins over an extensive range of vessel size.
Details
- Title
- Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy
- Authors/Creators
- J.P. Ngo (Author/Creator) - Monash UniversityB. Le (Author/Creator) - Monash UniversityZ. Khan (Author/Creator) - The University of Western AustraliaM.M. Kett (Author/Creator) - Monash UniversityB.S. Gardiner (Author/Creator) - Murdoch UniversityD.W. Smith (Author/Creator) - The University of Western AustraliaM.M. Melhem (Author/Creator) - Monash UniversityA. Maksimenko (Author/Creator) - Australian SynchrotronJ.T. Pearson (Author/Creator) - Monash UniversityR.G. Evans (Author/Creator) - Monash University
- Publication Details
- Clinical and Experimental Pharmacology and Physiology, Vol.44(12), pp.1241-1253
- Publisher
- Wiley
- Identifiers
- 991005540782907891
- Copyright
- © 2017 John Wiley & Sons Australia, Ltd
- Murdoch Affiliation
- School of Engineering and Information Technology
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.55 Urology & Nephrology - General
- 1.55.830 Acute Kidney Injury
- Web Of Science research areas
- Pharmacology & Pharmacy
- Physiology
- ESI research areas
- Pharmacology & Toxicology