Journal article
MicroRNA-221 may be involved in lipid metabolism in mammary epithelial cells
The International Journal of Biochemistry & Cell Biology, Vol.97, pp.118-127
2018
Abstract
Milk lipids, important for infant growth and development, are produced and secreted by mammary gland under the regulation of steroid hormones, growth factors, and microRNAs (miRNAs). miR-221 has been identified in milk and adipocytes and it plays important roles in regulating normal mammary epithelial hierarchy and breast cancer stem cells; however, its roles in lipid metabolism in mammary epithelial cells (MECs), the cells of lipid synthesis and secretion, are as yet unknown. Through overexpression or inhibition of miR-221 expression, we found that it regulated lipid metabolism in MECs and was expressed differentially at various stages during murine mammary gland development. Inhibition of miR-221 expression increased lipid content in MECs through elevation of the lipid synthesis enzyme FASN, while overexpression of miR-221 reduced MEC lipid content. Moreover, the steroid hormones estradiol and progesterone decreased miR-221 expression with a subsequent increase in lipid formation in MECs. The expression of miR-221 was lower during lactation, which suggests that it may be involved in milk production. Therefore, miR-221 might be a useful target for influencing milk lipid production.
Details
- Title
- MicroRNA-221 may be involved in lipid metabolism in mammary epithelial cells
- Authors/Creators
- M. Chu (Author/Creator)Y. Zhao (Author/Creator)S. Yu (Author/Creator)Y. Hao (Author/Creator)P. Zhang (Author/Creator)Y. Feng (Author/Creator)H. Zhang (Author/Creator)D. Ma (Author/Creator)J. Liu (Author/Creator)M. Cheng (Author/Creator)L. Li (Author/Creator)W. Shen (Author/Creator)H. Cao (Author/Creator)Q. Li (Author/Creator)L. Min (Author/Creator)
- Publication Details
- The International Journal of Biochemistry & Cell Biology, Vol.97, pp.118-127
- Publisher
- Elsevier Limited
- Identifiers
- 991005544411307891
- Copyright
- © 2018 Elsevier Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.68 MicroRNA in Cancer
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- ESI research areas
- Biology & Biochemistry