Microcosm experiment investigating climate-induced thermal effects on human virus viability in seawater: qPCR vs capsid integrity for enhanced risk management

Jessica L. Kevill; Xiaorong Li; Alvaro Garcia-Delgado; Kate Herridge; Kata Farkas; William Gaze; Peter Robins; Shelagh K. Malham; Davey L. Jones

doi:10.1016/j.marpolbul.2024.117006

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Microcosm experiment investigating climate-induced thermal effects on human virus viability in seawater: qPCR vs capsid integrity for enhanced risk management

Journal article

Open access

Peer reviewed

Microcosm experiment investigating climate-induced thermal effects on human virus viability in seawater: qPCR vs capsid integrity for enhanced risk management

Jessica L. Kevill, Xiaorong Li, Alvaro Garcia-Delgado, Kate Herridge, Kata Farkas, William Gaze, Peter Robins, Shelagh K. Malham and Davey L. Jones

Marine pollution bulletin, Vol.208, 117006

2024

DOI: https://doi.org/10.1016/j.marpolbul.2024.117006

PMID: 39342910

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Abstract

Bathing water quality

Coastal pollution

Public health risk

Viral persistence

Wastewater discharge

Climate change is intensifying extreme weather events in coastal areas, leading to more frequent discharge of untreated wastewater containing human viruses into coastal waters. This poses a health risk, especially during heatwaves when bathing activity increases. A study examined the survival and viability of seven common wastewater viruses in seawater at different temperatures. Viral genomes were quantified using direct qPCR, whilst viability was assessed using Capsid Integrity qPCR. Results showed that T90 values from direct qPCR were much higher than those from CI-qPCR, suggesting that risk mitigation should be based on viral integrity tests. All viruses remained potentially viable for at least 72 h in environmental seawater and longer in sterile artificial seawater, highlighting the importance of biotic processes in viral inactivation. Viral persistence decreased with increasing temperature. Whilst heatwaves may partially reduce risks from human viral pathogens in coastal waters, they do not eliminate them entirely. [Display omitted]

Details

Title: Microcosm experiment investigating climate-induced thermal effects on human virus viability in seawater: qPCR vs capsid integrity for enhanced risk management
Authors/Creators: Jessica L. Kevill - Bangor University
Xiaorong Li - Bangor University
Alvaro Garcia-Delgado - Bangor University
Kate Herridge - Bangor University
Kata Farkas - Bangor University
William Gaze - University of Exeter
Peter Robins - Bangor University
Shelagh K. Malham - Bangor University
Davey L. Jones - Bangor University
Publication Details: Marine pollution bulletin, Vol.208, 117006
Publisher: Elsevier Ltd
Number of pages: 13
Grant note: BlueAdapt ProjectEuropean Union: 101057764 UKRI/HM GovernmentHorizon Europe - Pillar II: 101057764
This research has been funded through the BlueAdapt Project (www.blueadapt.eu, accessed on 26/12/2023) . BlueAdapt has received funding from the European Union's Horizon Europe research and innovation programme under grant agreement No 101057764 and by the UKRI/HM Government.
Identifiers: 991005707063107891
Murdoch Affiliation: Centre for Sustainable Farming Systems
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.710 Enteric Viruses
Web Of Science research areas: Environmental Sciences; Marine & Freshwater Biology
ESI research areas: Environment/Ecology