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Published (Version of Record)CC BY V4.0, Open Access
Journal article
Microevolution associated with clonal expansion of a hypervirulent, penicillin-resistant lineage of Neisseria meningitidis in Western Australia
Microbial genomics, Vol.11(11), 001530
2025
PMID: 41212612
Abstract
Neisseria meningitidis is a colonizer of the human nasopharynx which occasionally causes invasive meningococcal disease. Despite numerous reports of penicillin-resistant isolates, antimicrobial-resistant meningococcal clones have historically not persisted over long time periods or become globally distributed, presumably due to the imposed fitness cost associated with antimicrobial resistance. One exception is a penicillin-resistant clade of serogroup W clonal complex 11 (MenW:cc11) isolates identified in Western Australia in 2013, which has since caused disease globally. Here, we investigated the genomic changes associated with penicillin resistance in MenW:cc11 isolated during the 2013–2020 Western Australian meningococcal outbreak. Seventy-six MenW:cc11 disease-causing isolates underwent short-read whole genome sequencing. Reference genomes were generated for three isolates. In accordance with previous analysis, two phylogenetically distinct clusters were identified: cluster A (12 penicillin-susceptible isolates) and cluster B (63 penicillin-resistant isolates). Genomic comparison of the cluster A and cluster B isolates revealed 128 allelic differences present at the branching point between the two lineages. The differences included polymorphisms in genes associated with cell wall regulation, pilus biogenesis and the MtrR transcriptional regulator. A further 60 allelic changes were identified in the Western Australian isolates which were not identified in globally distributed cluster B isolates. In a search of the PubMLST Neisseria database, all allelic variants associated with the emergence of cluster B were found exclusively in other hypervirulent lineages. Taken together, the data suggest the global success of the penicillin-resistant N. meningitidis is due to compensatory mutations acquired through horizontal exchange from other hypervirulent lineages.
Details
- Title
- Microevolution associated with clonal expansion of a hypervirulent, penicillin-resistant lineage of Neisseria meningitidis in Western Australia
- Authors/Creators
- August Mikucki - The Kids Research Institute AustraliaEng Guan Chua - Marshall Centre for Infectious Diseases, Research and Training, School of Biomedical Sciences, University of Western Australia, Perth, WA, AustraliaChin Yen Tay - Marshall Centre for Infectious Diseases, Research and Training, School of Biomedical Sciences, University of Western Australia, Perth, WA, AustraliaMichael J Wise - The University of Western AustraliaGeoffrey W Coombs - Murdoch UniversityDavid J Speers - Pathwest Laboratory MedicineShakeel Mowlaboccus - Fiona Stanley HospitalCharlene M Kahler - The University of Western Australia
- Publication Details
- Microbial genomics, Vol.11(11), 001530
- Publisher
- Microbiology Society
- Number of pages
- 13
- Identifiers
- 991005828072507891
- Copyright
- © 2025 The Authors
- Murdoch Affiliation
- Centre for Biosecurity and One Health; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.714 Neisseria/Haemophilus
- Web Of Science research areas
- Genetics & Heredity
- Microbiology
- ESI research areas
- Microbiology