Journal article
Microsatellites from Clarias batrachus and their polymorphism in seven additional catfish species
Molecular Ecology Notes, Vol.3(3), pp.465-468
2003
Abstract
We isolated 18 novel microsatellite loci from the walking catfish (Clarias batrachus), and examined their cross-amplification in seven additional catfish species from three families. Sixteen of the 18 microsatellites were polymorphic in the source species (allele number: 2–10/locus and expected heterozygosity: 0.30–0.87). Moreover, nine of these 18 primer pairs cross-amplified specific and polymorphic products from the genome of at least six of the seven other catfish species tested. However, the success rate of cross-species amplification varied from locus to locus, indicating that cross-species amplification of microsatellites is locus-dependent.
Details
- Title
- Microsatellites from Clarias batrachus and their polymorphism in seven additional catfish species
- Authors/Creators
- G-H Yue (Author/Creator) - Temasek Life Sciences LaboratoryB. Kovács (Author/Creator) - Agricultural Biotechnology InstituteL. Orbán (Author/Creator) - Temasek Life Sciences Laboratory
- Publication Details
- Molecular Ecology Notes, Vol.3(3), pp.465-468
- Publisher
- Blackwell Publishing
- Identifiers
- 991005541606507891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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