Modulation and direction of the electrofusion response in plant protoplasts

M.J. Tempelaar; A. Duyst; S.Y. De Vlas; G. Krol; C. Symmonds; M.G.K. Jones

doi:10.1016/0168-9452(87)90136-1

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Modulation and direction of the electrofusion response in plant protoplasts

Journal article

Peer reviewed

Modulation and direction of the electrofusion response in plant protoplasts

M.J. Tempelaar, A. Duyst, S.Y. De Vlas, G. Krol, C. Symmonds and M.G.K. Jones

Plant Science, Vol.48(2), pp.99-105

1987

DOI: https://doi.org/10.1016/0168-9452(87)90136-1

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Abstract

In experiments on electrofusion of protoplasts (from Solanum brevidens, S. tuberosum, Nicotiana plumbaginifolia) the presence of divalent cations (Ca2+) at 1 mM in the fusion medium was found to increase the yield of hybrids observed directly after fusion and decrease the duration of pulse needed for fusion. Pretreatment of protoplasts with the polyamine spermine also enhanced fusion yield, and when combined with 1 mM Ca2+ the effects were additive. The improvement in fusion yield (2–4 fold) was most marked for protoplast populations (e.g. from suspension cultured cells) that were least responsive to electrofusion in mannitol alone. Short term viability, judged from FDA fluorescence was found to be high at these increased fusion levels. Optimum fusion parameters for electrofusion thus may be determined from short term experiments. Attempts to direct to fusion response between populations of protoplasts of identical properties by pretreatment of one fusion partner with spermine were inconclusive.

Details

Title: Modulation and direction of the electrofusion response in plant protoplasts
Authors/Creators: M.J. Tempelaar (Author/Creator) - University of Groningen
A. Duyst (Author/Creator) - University of Groningen
S.Y. De Vlas (Author/Creator) - University of Groningen
G. Krol (Author/Creator) - University of Groningen
C. Symmonds (Author/Creator) - The Hertz Corporation
M.G.K. Jones (Author/Creator) - The Hertz Corporation
Publication Details: Plant Science, Vol.48(2), pp.99-105
Publisher: Elsevier
Identifiers: 991005542181507891
Copyright: © 1987 Elsevier Ireland Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.119 Micropropagation
Web Of Science research areas: Biochemistry & Molecular Biology; Plant Sciences
ESI research areas: Plant & Animal Science