Journal article
Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly
Science, Vol.309(5733), pp.467-469
2005
Abstract
In Schizosaccharomyces pombe, the RNA interference (RNAi) machinery converts pericentromeric transcripts into small interfering RNAs (siRNAs) and is required for the assembly of pericentromeric heterochromatin. Here we describe a mutation in the second largest subunit of RNA polymerase II (RNAPII). Both wild-type and mutant RNAPII localized to the pericentromere. However, the mutation resulted in the loss of heterochromatic histone modifications and in the accumulation of pericentromeric transcripts, accompanied by the loss of siRNAs. This phenotype resembles mutants in RNAi and suggests that RNAPII couples pericentromeric transcription with siRNA processing and heterochromatin assembly.
Details
- Title
- Molecular Biology: RNA polymerase II is required for RNAi-dependent heterochromatin assembly
- Authors/Creators
- H. Kato (Author/Creator)D.B. Goto (Author/Creator)R.A. Martienssen (Author/Creator)T. Urano (Author/Creator)K. Furukawa (Author/Creator)Y. Murakami (Author/Creator)
- Publication Details
- Science, Vol.309(5733), pp.467-469
- Publisher
- American Association for the Advancement of Science
- Identifiers
- 991005545089307891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- 1.54 Molecular & Cell Biology - Genetics
- 1.54.100 Epigenetic Regulation
- Web Of Science research areas
- Biochemistry & Molecular Biology
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- Molecular Biology & Genetics