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Molecular and physiological alterations in chickpea under elevated CO2 concentrations
Journal article   Open access   Peer reviewed

Molecular and physiological alterations in chickpea under elevated CO2 concentrations

P. Palit, R. Ghosh, P. Tolani, A. Tarafdar, A. Chitikineni, P. Bajaj, M. Sharma, H. Kudapa and R.K. Varshney
Plant and Cell Physiology, Vol.61(8), pp.1449-1463
2020
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Abstract

The present study reports profiling of the elevated carbon dioxide (CO2) concentration responsive global transcriptome in chickpea, along with a combinatorial approach for exploring interlinks between physiological and transcriptional changes, important for the climate change scenario. Various physiological parameters were recorded in two chickpea cultivars (JG 11 and KAK 2) grown in open top chambers under ambient [380 parts per million (ppm)] and two stressed/elevated CO2 concentrations (550 and 700 ppm), at different stages of plant growth. The elevated CO2 concentrations altered shoot and root length, nodulation (number of nodules), total chlorophyll content and nitrogen balance index, significantly. RNA-Seq from 12 tissues representing vegetative and reproductive growth stages of both cultivars under ambient and elevated CO2 concentrations identified 18,644 differentially expressed genes including 9,687 transcription factors (TF). The differential regulations in genes, gene networks and quantitative real-time polymerase chain reaction (qRT-PCR) -derived expression dynamics of stress-responsive TFs were observed in both cultivars studied. A total of 138 pathways, mainly involved in sugar/starch metabolism, chlorophyll and secondary metabolites biosynthesis, deciphered the crosstalk operating behind the responses of chickpea to elevated CO2 concentration.

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Citation topics
3 Agriculture, Environment & Ecology
3.40 Forestry
3.40.1396 CO2 and Ozone Effects
Web Of Science research areas
Cell Biology
Plant Sciences
ESI research areas
Plant & Animal Science
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