Journal article
Molecular characterisation of a haplosporidian parasite infecting rock oysters Saccostrea cuccullata in north Western Australia
Journal of Invertebrate Pathology, Vol.95(1), pp.33-40
2007
Abstract
A haplosporidian parasite was identified in rock oysters (Saccostrea cuccullata Born, 1778) from the Montebello Islands (latitude -20.4'S longitude 115.53'E) off the northern coast of Western Australia by histopathological examination, PCR amplification and DNA sequencing of a segment of the SSU region of the parasite's rRNA gene. An oligonucleotide probe was constructed from the parasite's SSU rRNA gene in order to confirm its presence by in situ hybridisation. The parasite was disseminated throughout the gonad follicles of the host and to a lesser extent in the gills. The only parasite life stages thus far observed in this study were a uninucleate naked cell assumed to be a precursor to multinucleate plasmodial stages and a binucleate plasmodial stage. Whilst no parasite spores were detected in affected rock oysters, a phylogenetic analysis of the SSU region of the parasite's rRNA gene indicates the parasite belongs to the genus Minchinia. A PCR and in situ hybridisation assay for the Minchinia sp. was used to identify haplosporidians described by Hine and Thorne [Hine, P.M.., Thorne, T., 2002. Haplosporidium sp. (Haplosporidia: Haplosporidiidae) associated with mortalities among rock oysters Saccostrea cuccullata in north Western Australia. Dis. Aquat. Organ. 51, 123-13], in archived rock oyster tissues from the same coastline.
Details
- Title
- Molecular characterisation of a haplosporidian parasite infecting rock oysters Saccostrea cuccullata in north Western Australia
- Authors/Creators
- D. Bearham (Author/Creator) - Murdoch UniversityZ. Spiers (Author/Creator) - Murdoch UniversityS.R. Raidal (Author/Creator) - ScienceJ.B. Jones (Author/Creator) - Government of Western AustraliaP.K. Nicholls (Author/Creator) - Murdoch University
- Publication Details
- Journal of Invertebrate Pathology, Vol.95(1), pp.33-40
- Publisher
- Elsevier
- Identifiers
- 991005541151407891
- Copyright
- Crown Copyright © 2007. Published by Elsevier Inc.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- 3.2 Marine Biology
- 3.2.1002 Bivalve Ecology
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