Journal article
Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265
BMC Genomics, Vol.16(1)
2015
Abstract
Background
sdw1/denso is one of the most important and useful semi-dwarf genes in barley breeding. At least four sdw1/denso alleles have been reported and HvGA20ox 2 is considered as the candidate gene. Up to date, results of studies have not univocally proven the genetic relationship between sdw1/denso and HvGA20ox 2.
Results
In the present study, a complete deletion of Morex_contig_40861 including both HvGA20ox 2 and Mloc_56463 genes was identified at the sdw1 locus from a semi-dwarf mutant Riso no. 9265. Expression of the genes encoding gibberellin biosynthesis (HvGA20ox 1 and HvGA3ox 2 ) were increased in the mutant compared to the wild type Bomi, while the expression of GA catabolic gene HvGA2ox 3 was decreased. Over-expression of HvGA20ox 2 could rescue the semi-dwarf phenotype and increase GAs concentration.
Conclusions
We confirmed that a GA biosynthetic enzyme HvGA20ox 2 , acted as GA 20-oxidase, is the functional gene for the sdw1/denso semi-dwarfism. Lose of HvGA20ox 2 is partially compensated by HvGA20ox 1 and further feedback is regulated by gibberellin. We also deduced that the sdw1/denso allele itself affects later heading owing to its reduced endogenous GAs concentration.
Details
- Title
- Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265
- Authors/Creators
- Q. Jia (Author/Creator) - Zhejiang UniversityC. Li (Author/Creator) - Murdoch UniversityY. Shang (Author/Creator) - ZheJiang Academy of Agricultural SciencesJ. Zhu (Author/Creator) - ZheJiang Academy of Agricultural SciencesW. Hua (Author/Creator) - ZheJiang Academy of Agricultural SciencesJ. Wang (Author/Creator) - ZheJiang Academy of Agricultural SciencesJ. Yang (Author/Creator) - ZheJiang Academy of Agricultural SciencesG. Zhang (Author/Creator) - Zhejiang University
- Publication Details
- BMC Genomics, Vol.16(1)
- Publisher
- BioMed Central
- Identifiers
- 991005540124707891
- Copyright
- © 2015 Jia et al.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
110 File views/ downloads
49 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics