Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat

Z. Yu; Y. Peng; S. Islam; M. She; M. Lu; D. Lafiandra; N. Roy; A. Juhász; G. Yan; W. Ma

doi:10.1016/j.jcs.2019.01.003

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Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat

Journal article

Peer reviewed

Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat

Z. Yu, Y. Peng, S. Islam, M. She, M. Lu, D. Lafiandra, N. Roy, A. Juhász, G. Yan and W. Ma

Journal of Cereal Science, Vol.86, pp.9-14

2019

DOI: https://doi.org/10.1016/j.jcs.2019.01.003

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Abstract

An increased number of high molecular weight glutenin subunits (HMW-GSs) is advantageous to the formation of glutenin macropolymer, the major determinant of end-product quality. Out of the six HMW-GS genes, Glu-1Ay is typically inactive in most hexaploid wheat cultivars. In the current study, we characterized two active Glu-1Ay genes Glu-1AyN11 and Glu-1AyC422 from a hexaploid wheat cultivar N11 and a durum wheat cultivar C422, respectively. The molecular masses of the 1Ay subunit of N11 (Glu-1AyN11) and C422 (Glu-1AyC422) were 61.1 kDa and 63.9 kDa, respectively, measured by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Molecular characterization of Glu-1AyN11 and Glu-1AyC422 showed their complete coding regions were 1764 bp and 1827 bp in length, respectively. The deduced amino acid sequence of both Glu-1AyN11 and Glu-1AyC422 has a similar typical primary structure as the other y-type HMW-GSs, but in comparison to Glu-1By and Glu-1Dy subunits, the Glu-1Ay subunit has one less conserved cysteine residue in the repetitive domain. Phylogenetic analysis revealed that the inactive Glu-1Ay had a closer evolutionary relationship with Glu-1AyC422 than with Glu-1AyN11. The divergence time among Glu-1AyN11, Glu-1AyC422, and other y-type subunit genes was also estimated.

Details

Title: Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat
Authors/Creators: Z. Yu (Author/Creator) - Murdoch University
Y. Peng (Author/Creator) - Murdoch University
S. Islam (Author/Creator) - Murdoch University
M. She (Author/Creator) - Murdoch University
M. Lu (Author/Creator) - Australian Grain Technologies, PO Box 219, Narrabri, NSW, 2390, Australia
D. Lafiandra (Author/Creator) - Università degli Studi della Tuscia
N. Roy (Author/Creator) - Murdoch University
A. Juhász (Author/Creator) - Murdoch University
G. Yan (Author/Creator) - The University of Western Australia
W. Ma (Author/Creator) - Murdoch University
Publication Details: Journal of Cereal Science, Vol.86, pp.9-14
Publisher: Academic Press
Identifiers: 991005543166307891
Copyright: © 2019 Elsevier Ltd.
Murdoch Affiliation: State Agricultural Biotechnology Centre; School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Food Science & Technology
ESI research areas: Agricultural Sciences