Journal article
Molecular characterization of Giardia duodenalis in children in Kenya
BMC Infectious Diseases, Vol.16(1)
2016
Abstract
Background
Giardia duodenalis is an important intestinal protozoan in humans worldwide with high infection rates occurring in densely populated and low resource settings. The parasite has been recorded to cause diarrhea in children. This study was carried out to identify G. duodenalis assemblages and sub-assemblages in children presenting with diarrhea in Kenya.
Methods
A total of 2112 faecal samples were collected from children aged ≤5 years and screened for the presence of Giardia cysts using microscopy. A total of 96 (4.5 %) samples were identified as Giardia positive samples and were genotyped using glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and β-giardin loci.
Results
The three markers successfully genotyped 72 isolates and grouped 2 (1.4) isolates as Assemblage A, 64 (88.9) as Assemblage B and 7 (9.7 %) consisted of mixed infections with assemblage A and B. A further analysis of 50 isolates using GDH Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) categorized 2 assemblage A isolates as sub-assemblage AII while 6 and 14 assemblage B isolates were categorized into sub-assemblage BIII and BIV respectively. A mixed infection with sub-assemblage BIII and BIV was recorded in 28 isolates. Over half (55.6 %) of Giardia infections were recorded among the children between 13 to 48 months old.
Conclusion
This paper reports the first data on the assemblages and sub-assemblages of Giardia duodenalis in children representing with diarrhea in Kenya.
Details
- Title
- Molecular characterization of Giardia duodenalis in children in Kenya
- Authors/Creators
- C. Mbae (Author/Creator) - Kenya Medical Research InstituteE. Mulinge (Author/Creator) - Kenya Medical Research InstituteF. Guleid (Author/Creator) - Kenya Medical Research InstituteJ. Wainaina (Author/Creator) - International Livestock Research InstituteA. Waruru (Author/Creator) - Kenya Medical Research InstituteZ.K. Njiru (Author/Creator) - Murdoch UniversityS. Kariuki (Author/Creator) - Kenya Medical Research Institute
- Publication Details
- BMC Infectious Diseases, Vol.16(1)
- Publisher
- BioMed Central
- Identifiers
- 991005543811307891
- Copyright
- © Mbae et al. 2016
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
- Infectious Diseases
- ESI research areas
- Immunology