Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

K. Wang; X.L. An; L.P. Pan; K. Dong; L.Y. Gao; S.L. Wang; Z.Z. Xie; Z. Zhang; R. Appels; W. Ma; Y.M. Yan

doi:10.1111/j.1601-5223.2011.02215.x

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Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

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Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement

K. Wang, X.L. An, L.P. Pan, K. Dong, L.Y. Gao, S.L. Wang, Z.Z. Xie, Z. Zhang, R. Appels, W. Ma, …

Hereditas, Vol.149(1), pp.41-49

2012

DOI: https://doi.org/10.1111/j.1601-5223.2011.02215.x

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Abstract

Two x-type high molecular weight glutenin subunits (HMW-GS) in Aegilops tauschii, 1Dx3 t and 1Dx4 t were identified by SDS-PAGE and MALDI-TOF-MS. Their complete coding sequences were isolated by AS-PCR. 1Dx3 t and 1Dx4 t genes consist of 2535 bp and 2508 bp and encode 845 and 836 amino acid residues, respectively. The deduced molecular masses of 1Dx3 t and 1Dx4 t gene products are 87655.26 Da and 86664.24 Da, respectively, well corresponding to the molecular masses measured by MALDI-TOF-MS. A total of 18 SNPs were identified between 1Dx3 t and 1Dx4 t. Comparing with 1Dx5 subunit, 1Dx3 t had a six amino acid insertion at 146-151 while the 1Dx4 t had a nine amino acid deletion when compared with 1Dx3 t subunit. The authenticity of the cloned 1Dx3 t and 1Dx4 t genes were confirmed by successful expression of their ORFs in E. coli. Comparison and phylogenetic tree based on the amino acid and nucleotide sequences confirmed that 1Dx3 t was most closely related to 1Dx5 subunit that is widely accepted as a superior subunit for bread-making property. The secondary structure prediction demonstrated that 1Dx3 t subunit has significantly high α-helix and β-strand contents, suggesting it might have positive effects on dough quality.

Details

Title: Molecular characterization of HMW-GS 1Dx3t and 1Dx4t genes from Aegilops tauschii and their potential value for wheat quality improvement
Authors/Creators: K. Wang (Author/Creator) - Capital Normal University
X.L. An (Author/Creator) - Capital Normal University
L.P. Pan (Author/Creator) - Capital Normal University
K. Dong (Author/Creator) - Capital Normal University
L.Y. Gao (Author/Creator) - Capital Normal University
S.L. Wang (Author/Creator) - Capital Normal University
Z.Z. Xie (Author/Creator) - Capital Normal University
Z. Zhang (Author/Creator) - Capital Normal University
R. Appels (Author/Creator) - Capital Normal University
W. Ma (Author/Creator) - Capital Normal University
Y.M. Yan (Author/Creator) - Capital Normal University
Publication Details: Hereditas, Vol.149(1), pp.41-49
Publisher: : Blackwell Publishing Inc
Identifiers: 991005546349207891
Copyright: © 2012 The Authors.
Murdoch Affiliation: Centre for Comparative Genomics; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics