Journal article
Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species
BMC Plant Biology, Vol.12, Article number: 221
2012
Abstract
Background
Brachypodium distachyon L. is a newly emerging model plant system for temperate cereal crop species. However, its grain protein compositions are still not clear. In the current study, we carried out a detailed proteomics and molecular genetics study on grain glutenin proteins in B. distachyon.
Results
SDS-PAGE and RP-HPLC analysis of grain proteins showed that Brachypodium has few gliadins and high molecular weight glutenin subunits. In contrast the electrophoretic patterns for the albumin, globulin and low molecular weight glutenin subunit (LMW-GS) fractions of the grain protein were similar to those in wheat. In particular, the LMW-C type subunits in Brachypodium were more abundant than the equivalent proteins in common wheat. Southern blotting analysis confirmed that Brachypodium has 4–5 copies of LMW-GS genes. A total of 18 LMW-GS genes were cloned from Brachypodium by allele specific PCR. LMW-GS and 4 deduced amino acid sequences were further confirmed by using Western-blotting and MALDI-TOF-MS. Phylogenetic analysis indicated that Brachypodium was closer to Ae. markgrafii and Ae. umbellulata than to T. aestivum.
Conclusions
Brachypodium possessed a highly conserved Glu-3 locus that is closely related to Triticum and related species. The presence of LMW-GS in B. distachyon grains indicates that B. distachyon may be used as a model system for studying wheat quality attributes.
Details
- Title
- Molecular characterization of LMW-GS genes in Brachypodium distachyon L. reveals highly conserved Glu-3 loci in Triticum and related species
- Authors/Creators
- S. Wang (Author/Creator) - Capital Normal UniversityK. Wang (Author/Creator) - Institute of Crop SciencesG. Chen (Author/Creator) - Capital Normal UniversityD. Lv (Author/Creator) - Capital Normal UniversityX. Han (Author/Creator) - Capital Normal UniversityZ. Yu (Author/Creator) - Capital Normal UniversityX. Li (Author/Creator) - Capital Normal UniversityX. Ye (Author/Creator) - Institute of Crop SciencesS.L.K. Hsam (Author/Creator) - Technical University of MunichW. Ma (Author/Creator) - Agriculture and FoodR. Appels (Author/Creator) - Agriculture and FoodY. Yan (Author/Creator) - Capital Normal University
- Publication Details
- BMC Plant Biology, Vol.12, Article number: 221
- Publisher
- BioMed Central
- Identifiers
- 991005544103507891
- Copyright
- © 2012 Wang et al
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
- Note
- This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science