Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

M. Lebdah; D.S. Alshaya; A.S. Jalal; M.R. Mousa; M.M. Radwan; M. Samir; A. Adel; N.M. Albaqami; M.T. El-Saadony; K.A. El-Tarabily; Y.F.H. El basrey

doi:10.1016/j.psj.2022.101918

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Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Journal article

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Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

M. Lebdah, D.S. Alshaya, A.S. Jalal, M.R. Mousa, M.M. Radwan, M. Samir, A. Adel, N.M. Albaqami, M.T. El-Saadony, K.A. El-Tarabily, …

Poultry Science, Vol.101(12), Art. 101918

2022

DOI: https://doi.org/10.1016/j.psj.2022.101918

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Abstract

Inclusion body hepatitis (IBH) is an economically significant viral disease that primarily affects broiler chickens. At least 12 different aviadenovirus serotypes are responsible for causing IBH. This study aimed to use polymerase chain reaction (PCR) and phylogenetic analysis to characterize fowl adenovirus isolates that were in circulation from 2019 to 2021 and investigate the pathogenicity of the isolated strains in commercial broiler chickens. Suspected liver samples were molecularly identified using hexon gene targeting by PCR, and viruses were isolated using chick embryo liver cell culture. For serotype identification, the fowl adenovirus-positive samples were subjected to hexon gene sequencing and phylogenetic analysis. The pathogenicity of two isolates was tested in commercial chickens via the oral route. The phylogenetic analysis of the hexon gene showed that the isolated viruses clustered with serotype 8a species E. On testing the pathogenicity of the isolates based on necropsy and histopathological examination, no mortality was observed; however, lesions were observed in the liver, kidney, heart, pancreas, bursa, and lung specimens with intermittent virus shedding at different time points throughout the experimental period. Further research on the likelihood of vaccine production is warranted to limit disease-related losses.

Details

Title: Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens
Authors/Creators: M. Lebdah (Author/Creator) - Zagazig University
D.S. Alshaya (Author/Creator) - Princess Nourah bint Abdulrahman University
A.S. Jalal (Author/Creator) - Princess Nourah bint Abdulrahman University
M.R. Mousa (Author/Creator) - Cairo University
M.M. Radwan (Author/Creator) - Cairo University
M. Samir (Author/Creator) - Animal Health Research Institute
A. Adel (Author/Creator) - Animal Health Research Institute
N.M. Albaqami (Author/Creator) - King Abdulaziz University
M.T. El-Saadony (Author/Creator) - Zagazig University
K.A. El-Tarabily (Author/Creator)
Y.F.H. El basrey (Author/Creator) - Zagazig University
Publication Details: Poultry Science, Vol.101(12), Art. 101918
Publisher: Poultry Science Association
Identifiers: 991005542212107891
Copyright: © 2022 The Authors.
Murdoch Affiliation: Harry Butler Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.161 Virology - Identification & Sequencing; 1.161.1756 Avian Viral Diseases
Web Of Science research areas: Agriculture, Dairy & Animal Science
ESI research areas: Plant & Animal Science