Journal article
Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract
Applied and Environmental Microbiology, Vol.78(19), pp.6799-6803
2012
Abstract
The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n=146) isolated from different regions of the intestinal tracts of eight pigs (dueodenum, ileum, colon, and feces). The isolates were typed using the method of random amplified polymorphic DNA (RAPD) and screened for the presence of bacteriocin genes and plasmid replicon types. Molecular analysis of variance using the RAPD data showed that E. coli isolates are nonrandomly distributed among different gut regions, and that gut region accounted for 25% (P<0.001) of the observed variation among strains. Bacteriocin screening revealed that a bacteriocin gene was detected in 45% of the isolates, with 43% carrying colicin genes and 3% carrying microcin genes. Of the bacteriocins observed (H47, E3, E1, E2, E7, Ia/Ib, and B/M), the frequency with which they were detected varied with respect to gut region for the colicins E2, E7, Ia/Ib, and B/M. The plasmid replicon typing gave rise to 25 profiles from the 13 Inc types detected. Inc F types were detected most frequently, followed by Inc HI1 and N types. Of the Inc types detected, 7 were nonrandomly distributed among isolates from the different regions of the gut. The results of this study indicate that not only may the different regions of the gastrointestinal tract harbor different strains of E. coli but also that strains from different regions have different characteristics.
Details
- Title
- Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract
- Authors/Creators
- S. Abraham (Author/Creator)D.M. Gordon (Author/Creator)J. Chin (Author/Creator)H.J.M. Brouwers (Author/Creator)P. Njuguna (Author/Creator)M.D. Groves (Author/Creator)R. Zhang (Author/Creator)T.A. Chapman (Author/Creator)
- Publication Details
- Applied and Environmental Microbiology, Vol.78(19), pp.6799-6803
- Publisher
- American Society for Microbiology
- Identifiers
- 991005542658207891
- Copyright
- 2012, American Society for Microbiology.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
29 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.42 Bacteriology
- 1.42.545 E. coli Pathogenesis
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Biology & Biochemistry