Journal article
Molecular characterization of haemaphysalis species and a molecular genetic key for the identification of haemaphysalis of North America
Frontiers in Veterinary Science, Vol.7, Art.141
2020
Abstract
Haemaphysalis longicornis (Acari: Ixodidae), the Asian longhorned tick, is native to East Asia, but has become established in Australia and New Zealand, and more recently in the United States. In North America, there are other native Haemaphysalis species that share similar morphological characteristics and can be difficult to identify if the specimen is damaged. The goal of this study was to develop a cost-effective and rapid molecular diagnostic assay to differentiate between exotic and native Haemaphysalis species to aid in ongoing surveillance of H. longicornis within the United States and help prevent misidentification. We demonstrated that restriction fragment length polymorphisms (RFLPs) targeting the 16S ribosomal RNA and the cytochrome c oxidase subunit I (COI) can be used to differentiate H. longicornis from the other Haemaphysalis species found in North America. Furthermore, we show that this RFLP assay can be applied to Haemaphysalis species endemic to other regions of the world for the rapid identification of damaged specimens. The work presented in this study can serve as the foundation for region specific PCR-RFLP keys for Haemaphysalis and other tick species and can be further applied to other morphometrically challenging taxa.
Details
- Title
- Molecular characterization of haemaphysalis species and a molecular genetic key for the identification of haemaphysalis of North America
- Authors/Creators
- A.T. Thompson (Author/Creator) - University of GeorgiaK. Dominguez (Author/Creator) - University of GeorgiaC.A. Cleveland (Author/Creator) - University of GeorgiaS.J. Dergousoff (Author/Creator) - Agriculture and Agri-Food CanadaK. Doi (Author/Creator) - Nippon Veterinary and Life Science UniversityR.C. Falco (Author/Creator) - New York State Department of HealthT. Greay (Author/Creator) - Murdoch UniversityP. Irwin (Author/Creator) - Murdoch UniversityL.R. Lindsay (Author/Creator) - Public Health Agency of CanadaJ. Liu (Author/Creator) - Hebei Normal UniversityT.N. Mather (Author/Creator) - University of Rhode IslandC.L. Oskam (Author/Creator) - Murdoch UniversityR.I. Rodriguez-Vivas (Author/Creator) - Universidad Autónoma de YucatánM.G. Ruder (Author/Creator) - University of GeorgiaD. Shaw (Author/Creator) - University of GeorgiaS.L. Vigil (Author/Creator) - University of GeorgiaS. White (Author/Creator) - University of GeorgiaM.J. Yabsley (Author/Creator) - University of Georgia
- Publication Details
- Frontiers in Veterinary Science, Vol.7, Art.141
- Publisher
- Frontiers Media S.A.
- Identifiers
- 991005541169907891
- Copyright
- © 2020 The Authors
- Murdoch Affiliation
- Vector and Waterborne Pathogens Research Group
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 1 Clinical & Life Sciences
- 1.258 Zoonotic Diseases
- 1.258.227 Tick-borne Pathogens
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science