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Author’s VersionCC BY-NC-ND V4.0, Open Access
Journal article
Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia
Parasitology International, Vol.65(3), pp.205-208
2016
Abstract
The quokka, Setonix brachyurus, is a vulnerable, small marsupial endemic to Western Australia. Blood samples were collected from quokkas from three different geographical locations; Two Peoples Bay, Bald Island and Rottnest Island. The overall prevalence of trypanosomes by nested PCR at the 18S ribosomal RNA gene was 57.3% (63/110) with prevalences of 91.4%, 85.3% and 4.9% respectively for Two Peoples Bay, Bald Island and Rottnest Island. Phylogenetic analysis conducted on 47 18S PCR positives identified two Trypanosoma copemani genotypes, with T. copemani genotype B, the most prevalent genotype infecting quokka populations from the three locations with an overall prevalence of 51.8% (24/47) compared to 34% for T. copemani genotype A (16/47). The overall prevalence of mixed T. copemani genotype A and B infections was 14.9% (7/47). Phylogenetic analysis of 26 quokka isolates at the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) locus, largely supported the 18S analysis but identified a mixed infection in one quokka isolate (Q4112-4117 from Two Peoples Bay). T. copemani genotype B has previously only been isolated from quokkas and the Gilbert's potoroo whereas T. copemani genotype A has a wide host range and may be pathogenic. Further work is required to determine the clinical impact of T. copemani on marsupial populations.
Details
- Title
- Molecular characterization of native Australian trypanosomes in quokka (Setonix brachyurus) populations from Western Australia
- Authors/Creators
- J.M. Austen (Author/Creator)A. Paparini (Author/Creator)S.A. Reid (Author/Creator)J.A. Friend (Author/Creator)W.G.F. Ditcham (Author/Creator)U. Ryan (Author/Creator)
- Publication Details
- Parasitology International, Vol.65(3), pp.205-208
- Publisher
- Elsevier BV
- Identifiers
- 991005544084107891
- Copyright
- © 2015 Elsevier Ireland Ltd.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 1 Clinical & Life Sciences
- 1.261 Parasitology - Trypanosoma & Leishmania
- 1.261.596 Trypanosoma Biology
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology