Journal article
Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi
Hereditas, Vol.145(2), pp.92-98
2008
Abstract
This paper reports cloning and characterisation of four novel low-molecular-weight glutenin subunit (LMW-GS) genes (designated as TzLMW-m2, TzLMW-m1, TdLMW-m1 and AlLMW-m2) from the genomic DNA of Triticum dicoccoides, T. zhukovskyi and Aegilops longissima. The coding regions of TzLMW-m2, TzLMW-m1, TdLMW-m1 and AlLMW-m2 were 1056 bp, 903 bp, 1056 bp and 1050 bp in length, encoding 350, 300, 350 and 348 amino acid residues, respectively. The deduced amino acid sequences showed that the four novel genes were classified as LMW-m types and the comparison results indicated that the four genes had a more similar structure and a higher level of homology with the LMW-m genes than the LMW-s and –i types genes. However, the first cysteine residue's positions of TzLMW-m2, TdLMW-m1 and AlLMW-m2 were different from the others. Moreover, AlLMW-m2, TdLMW-m1 and TzLMW-m2 all possessed a longer repetitive domain, which was considered to be associated with good quality of wheat. The secondary structure prediction revealed that the content of β-strand in AlLMW-m2 and TdLMW-m1 exceeded the positive control, suggesting that AlLMW-m2 and TdLMW-m1 should be considered as candidate genes that may have positive effect on dough quality. In order to investigate the evolutionary relationship of the novel genes with the other LMW-GSs, a phylogenetic tree was constructed. The results lead to a speculation that AlLMW-m2, TdLMW-m1 and TzLMW-m2 may be the middle types during the evolution of LMW-m and LMW-s.
Details
- Title
- Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi
- Authors/Creators
- C. Jiang (Author/Creator) - Anhui Agricultural UniversityY-H Pei (Author/Creator) - Qingdao Agricultural UniversityY. Zhang (Author/Creator) - Beijing Union UniversityX. Li (Author/Creator) - Capital Normal UniversityD. Yao (Author/Creator) - Anhui Agricultural UniversityY. Yan (Author/Creator) - Capital Normal UniversityW. Ma (Author/Creator) - Agriculture and FoodS.L.K. Hsam (Author/Creator) - Technical University of MunichF. J. Zeller (Author/Creator) - Technical University of Munich
- Publication Details
- Hereditas, Vol.145(2), pp.92-98
- Publisher
- Blackwell Publishing Inc
- Identifiers
- 991005545172907891
- Copyright
- 2008 The Authors
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics