Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus

S. Boulos; J.I. Rood

doi:10.1016/0378-1097(86)90189-8

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Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus

Journal article

Peer reviewed

Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus

S. Boulos and J.I. Rood

FEMS Microbiology Letters, Vol.33(1), pp.73-78

1986

DOI: https://doi.org/10.1016/0378-1097(86)90189-8

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Abstract

The fimbrial subunit gene from the benign type BBacteroides nodosus isolate AC/6 was cloned into theSphI site of the multicopy vector plasmid pUC19. FiveEscherichia coli recombinants that were positive in a colony immunoassay were shown, by Western transfer analysis, to produce an immunologically cross-reacting protein of identical molecular size to fimbrial subunits prepared fromB. nodosus AC/6. Restriction endonuclease analysis showed that 4 of the recombinant plasmids carried a 6.7 kbSphI fragment. Recloning experiments showed that the fimbrial subunit gene was located within a 2.5 kbEcoRI-SphI fragment and that there was aPstI site located within the structural gene or its regulatory region. These recombinant clones will prove useful for the construction of a multivalent recombinant vaccine for the control of ovine footrot.

Details

Title: Molecular cloning of the fimbrial subunit gene from a benign type B isolate of Bacteroides nodosus
Authors/Creators: S. Boulos (Author/Creator) - Murdoch University
J.I. Rood (Author/Creator) - Murdoch University
Publication Details: FEMS Microbiology Letters, Vol.33(1), pp.73-78
Publisher: Blackwell Publishing
Identifiers: 991005542076607891
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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