Molecular detection of highly pathogenic avian influenza H5N8 in commercial broiler chicken farms from 2019 to 2022

Ahmed Setta; Nahed Yehia; Amani Osman Shakak; Diana A Al-Quwaie; Aljohara M Al-Otaibi; Mohamed T El-Saadony; Khaled A El-Tarabily; Heba Salem

doi:10.1016/j.psj.2023.102639

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Molecular detection of highly pathogenic avian influenza H5N8 in commercial broiler chicken farms from 2019 to 2022

Journal article

Open access

Peer reviewed

Molecular detection of highly pathogenic avian influenza H5N8 in commercial broiler chicken farms from 2019 to 2022

Ahmed Setta, Nahed Yehia, Amani Osman Shakak, Diana A Al-Quwaie, Aljohara M Al-Otaibi, Mohamed T El-Saadony, Khaled A El-Tarabily and Heba Salem

Poultry science, Vol.102(6), 102639

2023

DOI: https://doi.org/10.1016/j.psj.2023.102639

PMID: 37104901

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Abstract

Animals

Chickens

Farms

Influenza A Virus, H5N8 Subtype - genetics

Influenza in Birds - epidemiology

Phylogeny

Poultry Diseases - epidemiology

Highly pathogenic avian influenza (HPAI) is a serious viral infection that causes massive economic losses in poultry. The current study investigated the HPAI virus prevalence in commercial broiler chicken flocks from 2019 to 2022. Organ samples, including trachea, cecal tonsils, spleen, brain, as well as tracheal and cloacal swabs, were harvested from 111 problematic broiler chicken flocks that suffered from variable mortalities accompanied with respiratory signs (103 H5-vaccinated and 8 nonvaccinated flocks) in Egypt during the observation duration. Molecular tools were used to analyze the samples, including real-time reverse transcription-polymerase chain reaction (rRT-PCR) and sequence analysis of some PCR positive strains. The results indicated that 24 flocks were positive for HPAI H5N8, representing 21.6%, with 22.3% (23/103) prevalence and 12.5% (1/8) detection in vaccinated and nonvaccinated flocks, respectively, and they were almost detected in the autumn and winter seasons. Phylogenetic evaluation of the hemagglutinin (HA) gene showed that the 6 Egyptian strains were clustered in clade 2.3.4.4b and allocated into 2 groups (I and II). The samples recovered in 2019 were clustered in new subgroup A, and samples recovered in 2020 to 2022 were clustered in new subgroup B with 10 nucleotide mutations (R72S, A83D, T140A). In conclusion, HPAI H5N8 is a serious threat even in vaccinated birds; to control such problems, periodic molecular monitoring with vaccine efficacy evaluation and the use of preventive strategies are recommended.

Details

Title: Molecular detection of highly pathogenic avian influenza H5N8 in commercial broiler chicken farms from 2019 to 2022
Authors/Creators: Ahmed Setta - Cairo University
Nahed Yehia - Agricultural Research Center
Amani Osman Shakak - Shendi University
Diana A Al-Quwaie - King Abdulaziz University
Aljohara M Al-Otaibi - Princess Nourah bint Abdulrahman University
Mohamed T El-Saadony - Zagazig University
Khaled A El-Tarabily - Murdoch University, Centre for Terrestrial Ecosystem Science and Sustainability
Heba Salem - Cairo University
Publication Details: Poultry science, Vol.102(6), 102639
Publisher: Elsevier Inc. on behalf of Poultry Science Association Inc.
Identifiers: 991005581769907891
Murdoch Affiliation: Harry Butler Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.104 Virology - General; 1.104.126 Influenza
Web Of Science research areas: Agriculture, Dairy & Animal Science
ESI research areas: Plant & Animal Science