Journal article
Molecular epidemiology of clinical and colonizing Methicillin-Resistant staphylococcus isolates in companion animals
Frontiers in Veterinary Science, Vol.8, Art. 620491
2021
Abstract
In this study, we aimed to investigate the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) clinical and colonizing isolates of dogs and cats to profile contributing factors associated with their isolation. Nasal and rectal samples were collected from dogs and cats between 2015 and 2017 to identify colonizing isolates. Clinical isolates collected between 2003 and 2016 were retrieved from a Queensland university veterinary diagnostic laboratory. All isolates were identified using standard microbiological and molecular methods and were characterized by whole genome sequencing. Phylogenetic relationships and differences in epidemiological factors were investigated. Seventy-two MRSP isolates out of 1,460 colonizing samples and nine MRSP clinical isolates were identified. No MRSA was isolated. ST496 and ST749 were the most commonly isolated sequence types with different SCCmec types. ST496 clones spread both along the coast and more inland where ST749 was more centered in Brisbane. The resistance and virulence factors differed significantly between the two sequence types. ST496 colonizing and clinical isolates were similarly multidrug resistant. The virulence genes of ST749 colonizing and clinical isolates were similar as both contained the gene nanB for sialidase. There were no differences in the individual and clinical factors between predominant sequence types. High levels of antimicrobial resistance occurred in the majority of isolates, which is of potential concern to human and veterinary health. The phylogenetic clustering of isolates from this study and others previously identified in countries, particularly New Zealand, with which Australia has high volume of pet movements could suggest the importation of clones, which needs further investigation.
Details
- Title
- Molecular epidemiology of clinical and colonizing Methicillin-Resistant staphylococcus isolates in companion animals
- Authors/Creators
- H. Rynhoud (Author/Creator) - The University of QueenslandB.M. Forde (Author/Creator) - The University of QueenslandS.A. Beatson (Author/Creator) - The University of QueenslandS. Abraham (Author/Creator) - Murdoch UniversityE. Meler (Author/Creator) - The University of QueenslandR.J. Soares Magalhães (Author/Creator)J.S. Gibson (Author/Creator) - The University of Queensland
- Publication Details
- Frontiers in Veterinary Science, Vol.8, Art. 620491
- Publisher
- Frontiers Media S.A.
- Identifiers
- 991005542071007891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Antimicrobial Resistance and Infectious Disease Laboratory
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
113 File views/ downloads
55 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.173 MRSA and VRE
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science