Molecular identification and prevalence of Isospora sp. in pigs in Western Australia using a PCR–RFLP assay

J. Johnson; B. Samarasinghe; R. Buddle; A. Armson; U. Ryan

doi:10.1016/j.exppara.2008.06.005

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Molecular identification and prevalence of Isospora sp. in pigs in Western Australia using a PCR–RFLP assay

Journal article

Peer reviewed

Molecular identification and prevalence of Isospora sp. in pigs in Western Australia using a PCR–RFLP assay

J. Johnson, B. Samarasinghe, R. Buddle, A. Armson and U. Ryan

Experimental Parasitology, Vol.120(2), pp.191-193

2008

DOI: https://doi.org/10.1016/j.exppara.2008.06.005

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Abstract

Little is known about the prevalence of Isospora in domestic pigs in Western Australia. A total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of Western Australia. Faecal samples were screened using a PCR-RFLP assay based on the ITS-1 rDNA locus. An overall prevalence of 10.4% (30/289) was identified. Isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-weaned animals. PCR-RFLP analysis confirmed the presence of Isospora suis in 86.7% of the positive Isospora isolates. Isospora was significantly associated with diarrhea and the findings of this study suggest that management factors such as cleaning practices, flooring types and stocking densities need to be investigated in the porcine host to find new and improved measures for control.

Details

Title: Molecular identification and prevalence of Isospora sp. in pigs in Western Australia using a PCR–RFLP assay
Authors/Creators: J. Johnson (Author/Creator)
B. Samarasinghe (Author/Creator)
R. Buddle (Author/Creator)
A. Armson (Author/Creator)
U. Ryan (Author/Creator)
Publication Details: Experimental Parasitology, Vol.120(2), pp.191-193
Publisher: Academic Press
Identifiers: 991005540511607891
Copyright: © 2008 Elsevier Inc.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences; School of Nursing and Midwifery
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis; 1.217.1890 Coccidiosis
Web Of Science research areas: Parasitology
ESI research areas: Microbiology