Abstract
Two main classes of interaction between soluble dietary fibres (SDFs), such as (1,3: 1,4)-beta-D-glucan (beta G) and arabinoxylan (AX) and bile salt (BS) or diluted porcine bile, were identified by C-13 NMR and small angle X-ray scattering (SAXS). Small chemical shift differences of BS NMR resonances were consistent with effective local concentration or dilution of BS micelles mostly by beta G, suggesting dynamic interactions; whilst the reduced line widths/intensities observed were mostly caused by wheat AX and the highest molecular size and concentrations of beta G. SAXS showed evidence of changes in beta G but not AX in the presence of BS micelles, at > 13 nm length scale consistent with molecular level interactions. Thus intermolecular interactions between SDF and BS depend on both SDF source and its molecular weight and may occur alone or in combination. (C) 2015 Elsevier Ltd. All rights reserved.
