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Author’s VersionCC BY-NC-ND V4.0, Open Access
Journal article
Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia
Veterinary Microbiology, Vol.201, pp.141-145
2017
Abstract
Q fever is an infectious disease with a global distribution caused by the intracellular bacterium, Coxiella burnetii, which has been detected in a large number of tick species worldwide, including the brown dog tick, Rhipicephalus sanguineus. Recent reports of a high seroprevalance of C. burnetii in Australian dogs, along with the identification of additional Coxiella species within R. sanguineus ticks, has prompted an investigation into the presence and identification of Coxiella species in R. sanguineus ticks in Australia. Using a combination of C. burnetii species-specific IS1111a transposase gene and Coxiella genus-specific 16S rRNA PCR assays, a Coxiella sp. was identified in 100% (n = 199) of R. sanguineus ticks analysed, and C. burnetii was not detected in any R. sanguineus ticks studied. Phylogenetic analysis of the 16S rRNA gene revealed the Coxiella sequences were closely related to Coxiella sp. identified previously in R. sanguineus and R. turanicus ticks overseas. This study illustrates the value of using genus specific PCR assays to detect previously unreported bacterial species. Furthermore, the presence of an additional Coxiella sp. in Australia requires further investigation into its potential for contributing to serological cross-reactions during Q fever testing.
Details
- Title
- Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia
- Authors/Creators
- C.L. Oskam (Author/Creator)A.W. Gofton (Author/Creator)T. L. Greay (Author/Creator)R. Yang (Author/Creator)S. Doggett (Author/Creator)U.M. Ryan (Author/Creator)P.J. Irwin (Author/Creator)
- Publication Details
- Veterinary Microbiology, Vol.201, pp.141-145
- Publisher
- Elsevier BV
- Identifiers
- 991005544960607891
- Copyright
- © 2017 Elsevier B.V.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.258 Zoonotic Diseases
- 1.258.2263 Coxiella Burnetii
- Web Of Science research areas
- Microbiology
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science