Journal article
Molecular marker-assisted backcrossing breeding: An example to transfer a thermostable β-amylase gene from wild barley
Molecular Breeding, Vol.38(5)
2018
Abstract
Molecular marker-assisted backcrossing (MABC) is widely recommended for transferring favorable alleles from a donor to an elite variety. The question remains whether MABC is an effective approach to developing a competitive commercial variety. Here, we illustrate the transfer of a thermostable β-amylase allele Sd3 from wild barley into a commercial barley variety Gairdner. The elite lines were chosen for the Regional Crop Variety Test that followed a standard conventional breeding process. The results demonstrated that the Sd3 allele not only increased enzyme thermostability but dramatically enhanced diastatic power, an important malting quality trait. The BC1F1 individuals had a fundamental impact on the comprehensive agronomic and quality traits of the final progenies, demonstrating the importance of screening at the early stage of backcrossing in MABC. There was sufficient genetic variation in the BC3F3 families to select other malting quality and agronomic traits. Ten individual breeding lines with improved β-amylase thermostability also had improved yields and grain plumpness. Three elite lines with improved malting quality and agronomic traits were selected to provide a parental line to incorporate the wild barley allele for breeding a commercial variety. A new strategy should be considered that uses marker-assisted selection and backcrossing to transfer a favorable allele from a wild parent.
Details
- Title
- Molecular marker-assisted backcrossing breeding: An example to transfer a thermostable β-amylase gene from wild barley
- Authors/Creators
- Y. Xu (Author/Creator) - Yangtze UniversityX-Q Zhang (Author/Creator) - Murdoch UniversityS. Harasymow (Author/Creator) - Department of Primary Industries and Regional DevelopmentS. Westcott (Author/Creator) - Murdoch UniversityW. Zhang (Author/Creator) - Yangtze UniversityC. Li (Author/Creator) - Murdoch University
- Publication Details
- Molecular Breeding, Vol.38(5)
- Publisher
- Springer Netherlands
- Identifiers
- 991005542868307891
- Copyright
- © Springer Science+Business Media B.V., part of Springer Nature 2018
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Plant & Animal Science