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Molecular mechanisms of HIV Type 1 prophylaxis failure revealed by Single-genome sequencing
Journal article   Open access   Peer reviewed

Molecular mechanisms of HIV Type 1 prophylaxis failure revealed by Single-genome sequencing

H. Li, L. Blair, Y. Chen, G. Learn, K. Pfafferott, M. John, T. Bhattacharya, B.H. Hahn, S. Mallal, G.M. Shaw, …
Journal of Infectious Diseases, Vol.208(10), pp.1598-1603
2013
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Abstract

Trials of HIV-1 pre- and post-exposure prophylaxis show promise. Here, we describe a novel strategy for deciphering mechanisms of prophylaxis failure that could improve therapeutic outcomes. A healthcare worker began antiretroviral prophylaxis immediately after a high-risk needlestick injury but nonetheless became viremic 11 weeks later. Single genome sequencing of plasma viral RNA identified 15 drug-sensitive transmitted/founder HIV-1 genomes responsible for productive infection. Sequences emanating from these genomes exhibited extremely low diversity, suggesting virus sequestration as opposed to low-level replication as the cause of breakthrough infection. Identification of transmitted/founder viruses allows for genome-wide assessment of molecular mechanisms of prophylaxis failure.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
1 Clinical & Life Sciences
1.66 HIV
1.66.46 HIV Pathogenesis
Web Of Science research areas
Immunology
Infectious Diseases
Microbiology
ESI research areas
Immunology
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