Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality

S. Wang; Z. Yu; M. Cao; X. Shen; N. Li; X. Li; W. Ma; H. Weißgerber; F. Zeller; S. Hsam; Y. Yan

doi:10.1371/journal.pone.0058947

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Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality

Journal article

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Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality

S. Wang, Z. Yu, M. Cao, X. Shen, N. Li, X. Li, W. Ma, H. Weißgerber, F. Zeller, S. Hsam, …

PLoS ONE, Vol.8(4), e58947

2013

DOI: https://doi.org/10.1371/journal.pone.0058947

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Abstract

A wheat cultivar "Chinese Spring" chromosome substitution line CS-1Sl(1B), in which the 1B chromosome was substituted by 1Sl from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1Sl×2.3* and 1Sly16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1Sl, making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat.

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Title: Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality
Authors/Creators: S. Wang (Author/Creator) - Capital Normal University
Z. Yu (Author/Creator) - Capital Normal University
M. Cao (Author/Creator) - Capital Normal University
X. Shen (Author/Creator) - Capital Normal University
N. Li (Author/Creator) - Capital Normal University
X. Li (Author/Creator) - Capital Normal University
W. Ma (Author/Creator) - State Agriculture Biotechnology Centre, Murdoch University, Western Australian Department of Agriculture and Food, Perth, Western Australia, Australia
H. Weißgerber (Author/Creator) - Technical University of Munich
F. Zeller (Author/Creator) - Technical University of Munich
S. Hsam (Author/Creator)
Y. Yan (Author/Creator) - Capital Normal University
Publication Details: PLoS ONE, Vol.8(4), e58947
Publisher: Public Library of Science
Identifiers: 991005541343307891
Copyright: © 2013 Wang et al.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Plant Sciences
ESI research areas: Agricultural Sciences