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Journal article
Molecular typing of Giardia duodenalis in humans in Queensland - first report of Assemblage E
Parasitology, Vol.144(9), pp.1154-1161
2017
Abstract
Little is known about the genetic diversity of the protozoan parasite, Giardia duodenalis, infecting humans in Queensland, Australia. The present study typed 88 microscopically Giardia-positive isolates using assemblage-specific primers at the triose phosphate isomerase (tpi) gene and sequenced a subset of isolates at the glutamate dehydrogenase (gdh) gene (n = 30) and tpi locus (n = 27). Using the tpi-assemblage specific primers, G. duodenalis assemblage A and assemblage B were detected in 50% (44/88) and 38·6% (34/88) of samples, respectively. Mixed infections with assemblages A and B were identified in 4·5% (4/88) and assemblage E was identified in 6·8% (6/88) of samples. Sequence analysis at the gdh and tpi loci also confirmed the presence of assemblage E in these isolates. Cyst numbers per gram of feces (g-1) were determined using quantitative polymerase chain reaction and of the isolates that were typed as assemblage E, cyst numbers ranged 13·8-68·3 × 106 cysts g-1. This is the first report of assemblage E in humans in Australia, indicating that in certain settings, this assemblage may be zoonotic
Details
- Title
- Molecular typing of Giardia duodenalis in humans in Queensland - first report of Assemblage E
- Authors/Creators
- A. Zahedi (Author/Creator) - Murdoch UniversityD. Field (Author/Creator) - Queensland GovernmentU. Ryan (Author/Creator) - Murdoch University
- Publication Details
- Parasitology, Vol.144(9), pp.1154-1161
- Publisher
- Cambridge University Press
- Identifiers
- 991005542631307891
- Copyright
- © Cambridge University Press 2017
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- 1.246 Diarrheal Diseases
- 1.246.985 Cryptosporidium
- Web Of Science research areas
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- Microbiology