Journal article
Molecular typing of Pseudomonas pseudomallei: Restriction fragment length polymorphisms of rRNA genes
Journal of Clinical Microbiology, Vol.31(3), pp.533-539
1993
Abstract
The aim of this study was to develop a typing scheme for Pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rRNA genes (ribotyping). BamHI restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to Escherichia coli 16S and 23S rRNAs. A chemiluminescent labelling and detection system was used to visualize bands. On the basis of patterns, the strains were classified into 22 different groups, with the largest containing 29 isolates. While most of the ribotypes were not exclusive to a particular source, some ribotypes were restricted to a particular geographic area or to either a human or a particular animal species. Application of the typing scheme to isolates of four independent outbreaks among animals showed that certain ribotypes predominated. The study demonstrated ribotyping to be a useful tool in epidemiological investigations of melioidosis.
Details
- Title
- Molecular typing of Pseudomonas pseudomallei: Restriction fragment length polymorphisms of rRNA genes
- Authors/Creators
- A.E. Lew (Author/Creator)P.M. Desmarchelier (Author/Creator)
- Publication Details
- Journal of Clinical Microbiology, Vol.31(3), pp.533-539
- Publisher
- American Society for Microbiology
- Identifiers
- 991005543068207891
- Copyright
- © 1993 by the American Society for Microbiology
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
Metrics
83 File views/ downloads
86 Record Views