Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

A.M. Moustafa; M.D. Bennett; J. Edwards; K. Azim; M.A. Mesaik; M.I. Choudhary; P. Pathanasophon; A. Worarach; Q. Ali; M. Abubakar; R. Anjum

doi:10.1016/j.rvsc.2013.07.003

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Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

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Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

A.M. Moustafa, M.D. Bennett, J. Edwards, K. Azim, M.A. Mesaik, M.I. Choudhary, P. Pathanasophon, A. Worarach, Q. Ali, M. Abubakar, …

Research in Veterinary Science, Vol.95(3), pp.986-990

2013

DOI: https://doi.org/10.1016/j.rvsc.2013.07.003

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Abstract

A comparative genetic study of 23 field isolates and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology.

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Title: Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis
Authors/Creators: A.M. Moustafa (Author/Creator) - Murdoch University
M.D. Bennett (Author/Creator) - Murdoch University
J. Edwards (Author/Creator) - Murdoch University
K. Azim (Author/Creator) - International Center for Chemical and Biological Sciences
M.A. Mesaik (Author/Creator) - International Center for Chemical and Biological Sciences
M.I. Choudhary (Author/Creator) - International Center for Chemical and Biological Sciences
P. Pathanasophon (Author/Creator) - National Institute of Animal Health
A. Worarach (Author/Creator) - National Institute of Animal Health
Q. Ali (Author/Creator) - National Veterinary Research Institute
M. Abubakar (Author/Creator) - National Veterinary Research Institute
R. Anjum (Author/Creator) - National Veterinary Research Institute
Publication Details: Research in Veterinary Science, Vol.95(3), pp.986-990
Publisher: Elsevier Ltd
Identifiers: 991005543206407891
Copyright: © 2013 Elsevier Ltd.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.1656 Pasteurella Multocida
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science