Journal article
Morphological and genetic characterization of the first Isospora species (I. lugensae n. sp.) from a Kerguelen petrel (Lugensa brevirostris)
Parasitology Research
2021
Abstract
A new coccidian species, Isospora lugensae n. sp., was described from a single Kerguelen petrel (Lugensa brevirostris). Sporulated oocysts (n = 25) were characterized as subspheroidal to ellipsoidal measuring 24–25 μm × 21–23 μm (24.8 × 22.2 μm) in length/width (L/W), respectively, with a ratio of 1.07–1.14 μm (1.12). They contained a bi-layered wall with a thickness of 0.8–1.2 μm (1.0) and the outer layer smooth, with c.2/3 of total thickness. The oocyst contained two polar granules with both micropyle and oocyst residuum absent. Ovoidal sporocysts (n = 25) measured 15–16 μm × 10–11 μm (15.7 × 10.8 μm) in L/W, with a ratio of 1.41–1.49 μm (1.46). A flattened to knob-like Stieda body was present (c.0.5 μm deep × 2.5 μm wide) as well as a rounded to trapezoidal sub-Stieda (c.1.5 μm deep × 3.0 μm wide); however, no para-Stieda body was detected. The sporocyst residuum was composed of scattered spherules of different sizes, while vermiform sporozoites contained a refractile body, nucleus and visible striations. Analysis of the full-length mitochrondrial (mtDNA) genome revealed 3 protein-coding genes, (CytB, COI and COIII), 18 LSU and 14 small subunit (SSU) rDNA fragments, without transfer RNA genes with a total length of 6257 bp. Phylogenetic analysis of genomic SSU ribosomal sequences indicated that Isospora lugensae n. sp. is genetically similar to Eimeria reichenowi, isolated from a red-crowned crane (Grus japonensis) from Japan, with a 96.6% homology. The mtDNA sequence is most similar to Isospora serinuse with a 95.8% genetic similarity. Based on morphological and molecular data, this isolate is a new species of coccidian parasite that to date has only been found in a Kerguelen petrel.
Details
- Title
- Morphological and genetic characterization of the first Isospora species (I. lugensae n. sp.) from a Kerguelen petrel (Lugensa brevirostris)
- Authors/Creators
- R. Yang (Author/Creator) - Murdoch UniversityB. Brice (Author/Creator) - Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia.Q. Liu (Author/Creator) - Murdoch UniversityB.P. Berto (Author/Creator) - Universidade Federal Rural do Rio de JaneiroJ. Austen (Author/Creator) - Murdoch UniversityU.M. Ryan (Author/Creator) - Murdoch University
- Publication Details
- Parasitology Research
- Publisher
- Springer Verlag
- Identifiers
- 991005541919407891
- Copyright
- © 2021 Springer Nature Switzerland AG.
- Murdoch Affiliation
- Australian National Phenome Centre; Harry Butler Institute; Health Futures Institute; Vector and Waterborne Pathogens Research Group
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis
- 1.217.1890 Coccidiosis
- Web Of Science research areas
- Parasitology
- ESI research areas
- Microbiology