Journal article
Multi-Donor longitudinal antibody repertoire sequencing reveals the existence of public antibody clonotypes in HIV-1 infection
Cell Host & Microbe, Vol.23(6), pp.845-854
2018
Abstract
Characterization of single antibody lineages within infected individuals has provided insights into the development of Env-specific antibodies. However, a systems-level understanding of the humoral response against HIV-1 is limited. Here, we interrogated the antibody repertoires of multiple HIV-infected donors from an infection-naive state through acute and chronic infection using next-generation sequencing. This analysis revealed the existence of “public” antibody clonotypes that were shared among multiple HIV-infected individuals. The HIV-1 reactivity for representative antibodies from an identified public clonotype shared by three donors was confirmed. Furthermore, a meta-analysis of publicly available antibody repertoire sequencing datasets revealed antibodies with high sequence identity to known HIV-reactive antibodies, even in repertoires that were reported to be HIV naive. The discovery of public antibody clonotypes in HIV-infected individuals represents an avenue of significant potential for better understanding antibody responses to HIV-1 infection, as well as for clonotype-specific vaccine development.
Details
- Title
- Multi-Donor longitudinal antibody repertoire sequencing reveals the existence of public antibody clonotypes in HIV-1 infection
- Authors/Creators
- Ian Setliff (Author/Creator)W.J. McDonnell (Author/Creator)N. Raju (Author/Creator)R.G. Bombardi (Author/Creator)A.A. Murji (Author/Creator)C. Scheepers (Author/Creator)R. Ziki (Author/Creator)C. Mynhardt (Author/Creator)B.E. Shepherd (Author/Creator)A.A. Mamchak (Author/Creator)N. Garrett (Author/Creator)S.A. Karim (Author/Creator)S.A. Mallal (Author/Creator)J.E. Crowe (Author/Creator)L. Morris (Author/Creator)I.S. Georgiev (Author/Creator)
- Publication Details
- Cell Host & Microbe, Vol.23(6), pp.845-854
- Publisher
- Elsevier Inc.
- Identifiers
- 991005541588707891
- Copyright
- © 2018 The Authors
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.452 Somatic Hypermutation
- Web Of Science research areas
- Microbiology
- Parasitology
- Virology
- ESI research areas
- Microbiology