Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes

C. Wielinga; U. Ryan; R.C. Thompson; P. Monis

doi:10.1016/j.ijpara.2010.11.007

Back

Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes

Journal article

Open access

Peer reviewed

Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes

C. Wielinga, U. Ryan, R.C. Thompson and P. Monis

International Journal for Parasitology, Vol.41(5), pp.495-503

2011

DOI: https://doi.org/10.1016/j.ijpara.2010.11.007

Files and links (2)

pdf

multi-locus_analysis_of_giardia_duodenalis.pdfDownload View

Author’s Version Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

Recent research concerning Giardia duodenalis has focused on resolving possible sub-assemblages within Assemblages A and B to better understand host-specific and zoonotic relationships. In the present study nine cloned, cultured, Assemblage B isolates were used to investigate the intra-Assemblage B substitution patterns of conserved (ssrDNA, ef, h2b, h4) and variable (tpi, gdh, bg) genes to assess their suitability for further application to sub-assemblage analyses. The resolution of each gene was found to be proportional to its substitution rate and for the genetically narrow sample set examined, the variable genes best represented the consensus phylogeny while the conserved genes only established fractions. However it was demonstrated that the spectra of conserved and variable genes were required to ensure accuracy of inferred phylogeny and it was therefore concluded that further research into sub-Assemblage B groups would require a mixture of conserved and variable genes for the multi-locus analyses of this genetically broad assemblage.

Details

Title: Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes
Authors/Creators: C. Wielinga (Author/Creator)
U. Ryan (Author/Creator)
R.C. Thompson (Author/Creator)
P. Monis (Author/Creator)
Publication Details: International Journal for Parasitology, Vol.41(5), pp.495-503
Publisher: Elsevier BV
Identifiers: 991005543303207891
Copyright: © 2011 Australian Society for Parasitology Inc.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

300 File views/ downloads

105 Record Views

42 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology
ESI research areas: Microbiology