Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae

T. La; N.D. Phillips; B.l. Harland; P. Wanchanthuek; M.I. Bellgard; D.J. Hampson

doi:10.1016/j.vetmic.2009.03.025

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Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae

Journal article

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Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae

T. La, N.D. Phillips, B.l. Harland, P. Wanchanthuek, M.I. Bellgard and D.J. Hampson

Veterinary Microbiology, Vol.138(3-4), pp.330-338

2009

DOI: https://doi.org/10.1016/j.vetmic.2009.03.025

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Abstract

The purpose of this study was to develop and apply a multilocus sequence typing (MLST) scheme to study the molecular epidemiology of Brachyspira hyodysenteriae, the aetiological agent of swine dysentery. Sequences of seven conserved genomic loci were examined in 111 B. hyodysenteriae strains. Fifty-eight of these previously had been analysed by multilocus enzyme electrophoresis (MLEE), and for some the results of pulsed field gel electrophoresis (PFGE), restriction endonuclease analysis (REA) and/or serotyping also were available. The discriminatory power of these methods was compared. The strains were divided into 67 sequence types (STs) and 46 amino acid types (AATs) by MLST. The Index of Association value was significantly different from zero, indication that the population was clonal. Eleven clonal complexes (Cc) comprising between 2 and 10 STs were recognised. A population snapshot based on AATs placed 77.5% of the isolates from 30 of the AATs into one major cluster. The founder type AAT9 included 13 strains from nine STs that were isolated in Australia, Sweden, Germany and Belgium, including one from a mallard. The MLST results were generally comparable to those produced by MLEE. The MLST system had a similar discriminatory power to PFGE, but was more discriminatory than REA, MLEE or serotyping. MLST data provided evidence for likely transmission of strains between farms, but also for the occurrence of temporal "micro-evolution" of strains on individual farms. Overall, the MLST system proved to be a useful new tool for investigating the molecular epidemiology and diversity of B. hyodysenteriae.

Details

Title: Multilocus sequence typing as a tool for studying the molecular epidemiology and population structure of Brachyspira hyodysenteriae
Authors/Creators: T. La (Author/Creator)
N.D. Phillips (Author/Creator)
B.l. Harland (Author/Creator)
P. Wanchanthuek (Author/Creator)
M.I. Bellgard (Author/Creator)
D.J. Hampson (Author/Creator)
Publication Details: Veterinary Microbiology, Vol.138(3-4), pp.330-338
Publisher: Elsevier BV
Identifiers: 991005545391407891
Copyright: © 2009 Elsevier B.V.
Murdoch Affiliation: Animal Research Institute; Centre for Comparative Genomics; School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.2445 Lawsonia Intracellularis
Web Of Science research areas: Microbiology; Veterinary Sciences
ESI research areas: Plant & Animal Science