Journal article
Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi
New Phytologist, Vol.234(2), pp.688-703
2022
Abstract
Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated.
We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling.
We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P.
We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.
Details
- Title
- Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi
- Authors/Creators
- S. Keyes (Author/Creator) - University of SouthamptonA. Veelen (Author/Creator)D. McKay Fletcher (Author/Creator)C. Scotson (Author/Creator) - University of SouthamptonN. Koebernick (Author/Creator) - University of SouthamptonC. Petroselli (Author/Creator) - University of SouthamptonK. Williams (Author/Creator) - University of SouthamptonS. Ruiz (Author/Creator) - University of SouthamptonL. Cooper (Author/Creator) - University of SouthamptonR. Mayon (Author/Creator) - University of SouthamptonS. Duncan (Author/Creator) - University of SouthamptonM. Dumont (Author/Creator) - University of SouthamptonI. Jakobsen (Author/Creator) - University of CopenhagenG. Oldroyd (Author/Creator) - University of CambridgeA. Tkacz (Author/Creator) - University of OxfordP. Poole (Author/Creator) - University of OxfordF. Mosselmans (Author/Creator) - Diamond Light SourceC. Borca (Author/Creator) - Swiss Light Source, Forschungsstrasse 111, 5232, Villigen PSI, Switzerland.T. Huthwelker (Author/Creator) - Swiss Light Source, Forschungsstrasse 111, 5232, Villigen PSI, Switzerland.D.L. Jones (Author/Creator) - Bangor UniversityT. Roose (Author/Creator)
- Publication Details
- New Phytologist, Vol.234(2), pp.688-703
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005543565307891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.488 Mycorrhizal Symbiosis
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science