Journal article
Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples
Archives of Virology, Vol.157(2), pp.271-284
2012
Abstract
RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus,Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays.
Details
- Title
- Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples
- Authors/Creators
- S.J. Wylie (Author/Creator)H. Luo (Author/Creator)H. Li (Author/Creator)M.G.K. Jones (Author/Creator)
- Publication Details
- Archives of Virology, Vol.157(2), pp.271-284
- Publisher
- Springer Verlag
- Identifiers
- 991005540329407891
- Copyright
- © 2011 Springer-Verlag
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.267 Virology - Plant
- 3.267.356 Plant Virus Interactions
- Web Of Science research areas
- Virology
- ESI research areas
- Microbiology