Journal article
Multiplex-PCR typing of high molecular weight glutenin alleles in wheat
Euphytica, Vol.134(1), pp.51-60
2003
Abstract
In Australian commercial cultivars, each high molecular weight glutenin (Glu-1) homoeologous locus consists of one of two predominant alleles: Glu-A1a (subunit Ax1) or Glu-A1b (subunit Ax2*) at the GluA1 locus, Glu-B1b (Bx7 and By8 subunits) or Glu-B1i (Bx17 and By18 subunits) at the Glu-B1 locus, and Glu-D1d (Dx5 and Dy10 subunits) or Glu-D1a (Dx2 and Dy12 subunits) at the Glu-D1 locus. PCR-based assays have been developed in this study to discriminate between these common alleles at each locus. Primers specific for the Glu-A1 Ax2* gene give a single fragment of 1319 bp only in the presence of this gene. Primers targeting the Glu-B1 locus resulted in a co-dominant marker for which the Bx7 genotype produced two fragments (630 bp and 766 bp) and the Bx17 genotype a single fragment (669 bp). The third pair of primers was specific for the Dx5 gene and resulted in a single band of 478 bp. A multiplexed PCR assay was established which permitted the discrimination of the major HMW glutenins in a single PCR reaction and agarose gel assay. As the HMW glutenin composition of a wheat line is extremely important in determining the functional properties of wheat gluten, these markers are useful for the purposes of marker-assisted breeding. These markers may also be useful for the purpose of DNA-based identification of wheat varieties.
Details
- Title
- Multiplex-PCR typing of high molecular weight glutenin alleles in wheat
- Authors/Creators
- W. Ma (Author/Creator) - Plant IndustryW. Zhang (Author/Creator) - Plant IndustryK.R. Gale (Author/Creator) - Plant Industry
- Publication Details
- Euphytica, Vol.134(1), pp.51-60
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005541408007891
- Copyright
- 2003 Kluwer Academic Publishers
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Agronomy
- Horticulture
- Plant Sciences
- ESI research areas
- Plant & Animal Science