Journal article
Murine precision-cut liver slices as an Ex Vivo model of liver biology
Journal of Visualized Experiments, (157)
2020
Abstract
Understanding the mechanisms of liver injury, hepatic fibrosis, and cirrhosis that underlie chronic liver diseases (i.e., viral hepatitis, non-alcoholic fatty liver disease, metabolic liver disease, and liver cancer) requires experimental manipulation of animal models and in vitro cell cultures. Both techniques have limitations, such as the requirement of large numbers of animals for in vivo manipulation. However, in vitro cell cultures do not reproduce the structure and function of the multicellular hepatic environment. The use of precision-cut liver slices is a technique in which uniform slices of viable mouse liver are maintained in laboratory tissue culture for experimental manipulation. This technique occupies an experimental niche that exists between animal studies and in vitro cell culture methods. The presented protocol describes a straightforward and reliable method to isolate and culture precision-cut liver slices from mice. As an application of this technique, ex vivo liver slices are treated with bile acids to simulate cholestatic liver injury and ultimately assess the mechanisms of hepatic fibrogenesis.
Details
- Title
- Murine precision-cut liver slices as an Ex Vivo model of liver biology
- Authors/Creators
- M.A. Pearen (Author/Creator) - QIMR Berghofer Medical Research InstituteH.K. Lim (Author/Creator)F.D. Gratte (Author/Creator) - Curtin UniversityM.A. Fernandez-Rojo (Author/Creator) - Madrid Institute for Advanced StudiesS.K. Nawaratna (Author/Creator) - Griffith UniversityG.N. Gobert (Author/Creator) - Queen's University BelfastJ.K. Olynyk (Author/Creator) - Edith Cowan UniversityJ.E.E. Tirnitz-Parker (Author/Creator) - Curtin UniversityG.A. Ramm (Author/Creator) - QIMR Berghofer Medical Research Institute
- Publication Details
- Journal of Visualized Experiments, (157)
- Publisher
- MYJoVE Corporation
- Identifiers
- 991005545999707891
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.132 Extracellular Matrix & Cell Differentiation
- 1.132.306 TGF-Beta
- Web Of Science research areas
- Gastroenterology & Hepatology
- ESI research areas
- Clinical Medicine