Myeloid leukemia factor 1 associates with a novel heterogeneous nuclear ribonucleoprotein U-like molecule

L.N. Winteringham; R. Endersby; S. Kobelke; R.K. McCulloch; J.H. Williams; J. Stillitano; S.M. Cornwall; E. Ingley; S.P. Klinken

doi:10.1074/jbc.M605401200

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Myeloid leukemia factor 1 associates with a novel heterogeneous nuclear ribonucleoprotein U-like molecule

Journal article

Open access

Peer reviewed

Myeloid leukemia factor 1 associates with a novel heterogeneous nuclear ribonucleoprotein U-like molecule

L.N. Winteringham, R. Endersby, S. Kobelke, R.K. McCulloch, J.H. Williams, J. Stillitano, S.M. Cornwall, E. Ingley and S.P. Klinken

Journal of Biological Chemistry, Vol.281(50), pp.38791-38800

2006

DOI: https://doi.org/10.1074/jbc.M605401200

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Abstract

Myeloid leukemia factor 1 (MLF1) is an oncoprotein associated with hemopoietic lineage commitment and acute myeloid leukemia. Here we show that Mlf1 associated with a novel binding partner, Mlf1-associated nuclear protein (Manp), a new heterogenous nuclear ribonucleoprotein (hnRNP) family member, related to hnRNP-U. Manp localized exclusively in the nucleus and could redirect Mlf1 from the cytoplasm into the nucleus. The nuclear content of Mlf1 was also regulated by 14-3-3 binding to a canonical 14-3-3 binding motif within the N terminus of Mlf1. Significantly Mlf1 contains a functional nuclear export signal and localized primarily to the nuclei of hemopoietic cells. Mlf1 was capable of binding DNA, and microarray analysis revealed that it affected the expression of several genes, including transcription factors. In summary, this study reveals that Mlf1 translocates between nucleus and cytoplasm, associates with a novel hnRNP, and influences gene expression.

Details

Title: Myeloid leukemia factor 1 associates with a novel heterogeneous nuclear ribonucleoprotein U-like molecule
Authors/Creators: L.N. Winteringham (Author/Creator) - The University of Western Australia
R. Endersby (Author/Creator) - The University of Western Australia
S. Kobelke (Author/Creator) - UWA Centre for Medical Research
R.K. McCulloch (Author/Creator) - The University of Western Australia
J.H. Williams (Author/Creator) - The University of Western Australia
J. Stillitano (Author/Creator) - The University of Western Australia
S.M. Cornwall (Author/Creator) - The University of Western Australia
E. Ingley (Author/Creator) - School of Medicine and Pharmacology, UWA Centre for Medical Research
S.P. Klinken (Author/Creator) - UWA Centre for Medical Research
Publication Details: Journal of Biological Chemistry, Vol.281(50), pp.38791-38800
Publisher: American Society for Biochemistry and Molecular Biology Inc.
Identifiers: 991005541731107891
Copyright: © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.108 Molecular & Cell Biology - Cancer & Development; 1.108.579 Drosophila Development
Web Of Science research areas: Biochemistry & Molecular Biology
ESI research areas: Biology & Biochemistry