Natural bone collagen scaffold combined with autologous enriched bone marrow cells for induction of osteogenesis in an ovine spinal fusion model

Y. Qian; Z. Lin; J. Chen; Y. Fan; T. Davey; M. Cake; R. Day; K. Dai; J. Xu; M. Zheng

doi:10.1089/ten.tea.2009.0076

Back

Natural bone collagen scaffold combined with autologous enriched bone marrow cells for induction of osteogenesis in an ovine spinal fusion model

Journal article

Open access

Peer reviewed

Natural bone collagen scaffold combined with autologous enriched bone marrow cells for induction of osteogenesis in an ovine spinal fusion model

Y. Qian, Z. Lin, J. Chen, Y. Fan, T. Davey, M. Cake, R. Day, K. Dai, J. Xu and M. Zheng

Tissue Engineering Part A, Vol.15(11), pp.3547-3558

2009

DOI: https://doi.org/10.1089/ten.tea.2009.0076

Files and links (2)

pdf

natural_bone_collagen_scaffold.pdfDownload View

Published (Version of Record) Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

Autologous bone graft, the standard of bone grafting in achieving spinal fusion, is associated with several limitations and complications. The use of bone marrow cells (BMCs) as a potential cell source for spinal fusion, combined with a suitable scaffold to promote bone formation, may be a better choice. The aims of this study were to evaluate the efficacy of natural bone collagen scaffold (NBCS) combined with autologous-enriched BMCs for induction of osteogenesis in vitro and in vivo. Ovine-enriched BMCs were co-cultured with NBCS for 1, 2, 3, and 4 weeks to investigate whether NBCS would support the population expansion and differentiation of enriched BMCs. Using an ovine interbody fusion model, NBCS seeded with autologous enriched BMCs was implanted into the lumbar disc space. Fusion outcomes were compared with the use of the autograft, NBCS without BMCs, and BMCs without NBCS. In vitro results demonstrated that NBCS facilitated the population expansion and differentiation of ovine-enriched BMCs, promoting the expression of collagen type I and the formation of a mineralized matrix. The use of NBCS combined with enriched BMCs in vivo enhanced the spinal fusion rate (6 of 6 at 10 week) (p<0.05), the biomechanical stiffness of fusion masses, and bone volume at the fusion site (p<0.05). Histological findings also revealed that a combination of NBCS and BMCs induced new bone formation that integrated well with host bone tissue. In conclusion, NBCS is an effective scaffold that supports ovine-enriched BMCs. The combination of NBCS and BMCs may be a useful alternative for autograft in induction of spinal fusion.

Details

Title: Natural bone collagen scaffold combined with autologous enriched bone marrow cells for induction of osteogenesis in an ovine spinal fusion model
Authors/Creators: Y. Qian (Author/Creator) - School of Surgery
Z. Lin (Author/Creator) - School of Surgery
J. Chen (Author/Creator) - School of Surgery
Y. Fan (Author/Creator) - Perth Bone and Tissue Bank, Perth, Western Australia, Australia.
T. Davey (Author/Creator) - School of Surgery
M. Cake (Author/Creator) - Murdoch University
R. Day (Author/Creator) - The University of Western Australia
K. Dai (Author/Creator) - Shanghai Ninth People's Hospital
J. Xu (Author/Creator) - School of Surgery
M. Zheng (Author/Creator) - School of Surgery
Publication Details: Tissue Engineering Part A, Vol.15(11), pp.3547-3558
Publisher: Mary Ann Liebert
Identifiers: 991005541537907891
Copyright: © 2009 Mary Ann Liebert, Inc.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

317 File views/ downloads

36 Record Views

16 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.102 Stem Cell Research; 1.102.110 Mesenchymal Stem Cells
Web Of Science research areas: Cell & Tissue Engineering; Cell Biology; Engineering, Biomedical; Materials Science, Biomaterials
ESI research areas: Molecular Biology & Genetics