Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut

Z. Peng; H. Chen; L. Tan; H. Shu; R.K. Varshney; Z. Zhou; Z. Zhao; Z. Luo; A. Chitikineni; L. Wang; J. Maku; Y. López; M. Gallo; H. Zhou; J. Wang; M. Gifford

doi:10.1093/jxb/eraa505

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Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut

Journal article

Peer reviewed

Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut

Z. Peng, H. Chen, L. Tan, H. Shu, R.K. Varshney, Z. Zhou, Z. Zhao, Z. Luo, A. Chitikineni, L. Wang, …

Journal of Experimental Botany, Vol.72(4), pp.1104-1118

2020

DOI: https://doi.org/10.1093/jxb/eraa505

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Abstract

Microbial symbiosis in legumes is achieved through nitrogen-fixing root nodules, and these are important for sustainable agriculture. The molecular mechanisms underlying development of root nodules in polyploid legume crops are largely understudied. Through map-based cloning and QTL-seq approaches, we identified a pair of homoeologous GRAS transcription factor genes, Nodulation Signaling Pathway 2 (AhNSP2-B07 or Nb) and AhNSP2-A08 (Na), controlling nodulation in cultivated peanut (Arachis hypogaea L.), an allotetraploid legume crop, which exhibited non-Mendelian and Mendelian inheritance, respectively. The segregation of nodulation in the progeny of Nananbnb genotypes followed a 3:1 Mendelian ratio, in contrast to the 5:3~1:1 non-Mendelian ratio for nanaNbnb genotypes. Additionally, a much higher frequency of the nb allele (13%) than the na allele (4%) exists in the peanut germplasm collection, suggesting that Nb is less essential than Na in nodule organogenesis. Our findings reveal the genetic basis of naturally occurred non-nodulating peanut plants, which can be potentially used for nitrogen fixation improvement in peanut. Furthermore, the results have implications for and provide insights into the evolution of homoeologous genes in allopolyploid species.

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Title: Natural polymorphisms in a pair of NSP2 homoeologs can cause loss of nodulation in peanut
Authors/Creators: Z. Peng (Author/Creator) - University of Florida
H. Chen (Author/Creator) - South China Agricultural University
L. Tan (Author/Creator) - China Agricultural University
H. Shu (Author/Creator) - University of Florida
R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
Z. Zhou (Author/Creator) - South China Agricultural University
Z. Zhao (Author/Creator) - University of Florida
Z. Luo (Author/Creator) - University of Florida
A. Chitikineni (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
L. Wang (Author/Creator) - University of Florida
J. Maku (Author/Creator) - Glenville State College
Y. López (Author/Creator) - University of Florida
M. Gallo (Author/Creator) - Delaware Valley College
H. Zhou (Author/Creator) - University of Florida
J. Wang (Author/Creator) - University of Florida
M. Gifford (Author/Creator)
Publication Details: Journal of Experimental Botany, Vol.72(4), pp.1104-1118
Publisher: Oxford University Press
Identifiers: 991005544505107891
Copyright: © 2020 Oxford University Press
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.892 Rhizobium-Legume Symbiosis
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science