Journal article
Natural products show diverse mechanisms of action against Clostridium difficile
Journal of Applied Microbiology, Vol.126(2), pp.468-479
2018
Abstract
Aims: To investigate the mechanisms of action of natural products with bactericidal (cinnamon root powder, peppermint oil, trans-cinnamaldehyde, menthol and zingerone) or bacteriostatic (fresh garlic bulb extract, garlic clove powder, Leptospermum honey and allicin) activity against two Clostridium difficile strains.
Methods and Results: Bactericidal products significantly reduced intracellular ATP after 1 h (P ≤ 0·01), quantified using the BacTiter-Glo reagent, and damaged the cell membrane, shown by the leakage of both 260-nm-absorbing materials and protein, and the uptake of propidium iodide. Bacteriolysis was not observed, determined by measuring optical density of treated cell suspensions at 620-nm. The effect of three bacteriostatic products on protein synthesis was quantified using an Escherichia coli S30 extract system, with Leptospermum honey (16% w/v) showing significant inhibition (P < 0·01). Lastly, no products showed elevated minimum inhibitory concentrations against antimicrobial-resistant C. difficile, determined by broth microdilution.
Conclusions: Cytoplasmic membrane damage was identified as a mechanism of action that may contribute to the activity of several natural products against C. difficile.
Significance and Impact of the Study: This study describes the possible mechanisms of action of natural products against C. difficile, yet the efficacy in vivo to be determined.
Details
- Title
- Natural products show diverse mechanisms of action against Clostridium difficile
- Authors/Creators
- N. Roshan (Author/Creator)T.V. Riley (Author/Creator)D.R. Knight (Author/Creator)J.H. Steer (Author/Creator)K.A. Hammer (Author/Creator)
- Publication Details
- Journal of Applied Microbiology, Vol.126(2), pp.468-479
- Publisher
- Blackwell Publishing Ltd
- Identifiers
- 991005543767207891
- Copyright
- © 2018 The Society for Applied Microbiology
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
27 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.1133 Clostridium Infections
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Biology & Biochemistry