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Published (Version of Record)CC BY-NC-ND V4.0, Open Access
Journal article
Natural variations in a barley aldehyde oxidase 1 gene affect seed germination and malting quality
The Crop journal, Vol.13(1), pp.299-303
2025
Abstract
Multiple phytohormones, including gibberellin (GA), abscisic acid (ABA), and indole-3-acetic acid (IAA), regulate seed germination. In this study, a barley aldehyde oxidase 1 (HvAO1) gene was identified, which is located near the SD2 (seed dormancy 2) region at the telomeric end of chromosome 5H. A doubled-haploid population (AC Metcalfe/Baudin) was used to characterize HvAO1 and validated its association with seed germination and malting quality. Aldehyde oxidase is predicted to catalyse the oxidation of various aldehydes, such as indoleacetaldehyde and abscisic aldehyde, into IAA and ABA, which is the final step of IAA/ABA biogenesis. This process influences the final IAA/ABA concentration in the seed, affecting the seed dormancy. Sequence analysis revealed substantial variations in the HvAO1 promoter regions between AC Metcalfe and Baudin. The combining seed germination tests, genetic variation analysis, gene expression, and phytohormone measurements showed that Baudin, which displays strong seed dormancy, has a specific sequence variation in the promoter region of the HvAO1 gene. This variation is associated with a higher expression level of the HvAO1 gene and an increased level of ABA than those in AC Metcalfe, which shows weak dormancy and lacks this sequence variation. In addition to its strong effect on the SD2 gene, HvAO1 shows excellent potential to fine-tune malting quality and seed dormancy, as evidenced by genotyping with HvAO1-specific markers, dormancy phenotypes, and malting quality. Our findings provide a new strategy for introducing favourable HvAO1 alleles to achieve the desired level of seed dormancy and high malting quality in barley.
Details
- Title
- Natural variations in a barley aldehyde oxidase 1 gene affect seed germination and malting quality
- Authors/Creators
- Le Xu - Yangtze UniversityPeng Wang - Yangtze UniversityXiaoqi Zhang - Western Barley Genetic Alliance, Murdoch University, Perth, WA 6150, AustraliaQisen Zhang - Australian Export Grains Innovation CentrePenghao Wang - Murdoch UniversityYanhao Xu - Hubei Academy of Agricultural SciencesChengdao LiWenying Zhang
- Publication Details
- The Crop journal, Vol.13(1), pp.299-303
- Publisher
- KeAi Communications Co., Ltd
- Number of pages
- 5
- Grant note
- Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education: KFT202302 National Natural Science Foundation of China: 32372052
This work was supported by the Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education (KFT202302) and the National Natural Science Foundation of China (32372052) . We thank Dr. Tianhua He for assistance with revision.
- Identifiers
- 991005752198607891
- Copyright
- © 2024 Crop Science Society of China and Institute of Crop Science, CAAS.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Agronomy
- Plant Sciences
- ESI research areas
- Agricultural Sciences