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New Diagnostic and Bio-control tool for Trogoderma variabile (Ballion) larvae using (DI-SPME) Coupled (GC-MS)
Journal article   Open access

New Diagnostic and Bio-control tool for Trogoderma variabile (Ballion) larvae using (DI-SPME) Coupled (GC-MS)

Thamer Al-Shuwaili, Farhan Jasim Mohammed Al-Behadili, Mushtak Talib Mohammadali, Zainab Mohammed Khalaf, Beibei Li and Yonglin Ren
Tikrit Journal for Agricultural Sciences, Vol.24(4), pp.145-157
2024
DOI: https://doi.org/10.25130/tjas.24.4.12
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CC BY V4.0,  Open Access

Abstract

The objective of this study is to analyze Trogoderma variabile metabolism fed on various host grains (Rice, maize, wheat, oats, barley and canola) using GC-MS. Also, hydrocarbons profiling is suggested as a chemo-taxonomical marker/ tool to identify species of insect and especially when they are morphological quite similar like T. granarium. Six different commodities were the rearing substrates of T. variabile. To prepare the samples, insects were treated with acetonitrile. A method called DI-SPME was utilized, followed by GC-MS for analyzing and categorizing compounds. Furthermore, the specific type of grain that the insects fed on had a notable impact on the chemical compounds detected in Trogoderma variabile different larvae stages, including fatty acids and hydrocarbons. However, there were 17,16 , 14,16,14 and 13 compounds extracted form third larvae instar reared on barley, wheat,canola, maize, rice and oats respectively. in comparison to sixth larvae instar, results showed that there were 13,13,21,21,15 and 14 compounds from the larvae reared on oats and barley, wheat,canola, maize, rice and oats respectively.

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