New eSSR and gSSR markers added to Australian barley maps

K.L. Willsmore; P. Eckermann; R.K. Varshney; A. Graner; P. Langridge; M. Pallotta; J. Cheong; Kevin J. Williams

doi:10.1071/AR05384

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New eSSR and gSSR markers added to Australian barley maps

Journal article

Peer reviewed

New eSSR and gSSR markers added to Australian barley maps

K.L. Willsmore, P. Eckermann, R.K. Varshney, A. Graner, P. Langridge, M. Pallotta, J. Cheong and Kevin J. Williams

Australian Journal of Agricultural Research, Vol.57(9), pp.953-959

2006

DOI: https://doi.org/10.1071/AR05384

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Abstract

To enhance genetic maps of barley previously developed in Australia for identifying markers useable in molecular breeding, a new set of simple sequence repeat (SSR) and indel markers was added to the maps. These markers were developed through (i) database mining of barley expressed sequence tag (EST) sequences, (ii) comparative barley-rice genome analysis, and (iii) screening of a genomic library with SSR probes. The primer set selected for this study comprised 216 EST-SSR (eSSR) and 25 genomic SSR (gSSR) markers, which were screened for polymorphism on 4 doubled haploid (DH) or recombinant inbred line (RIL) populations. In total, 81 new markers were added to the maps, with good coverage on all 7 chromosomes, except 6H, which only had 2 new markers added. The marker order of previously published maps was re-evaluated by comparing recombination fractions calculated by 2 methods to discover the best position for each marker. The new SSR markers were then added to the updated maps. Several of these new markers are linked to important barley disease resistance genes such as those for cereal cyst nematode, spot form of net blotch, and leaf scald resistance, and are readily useable for marker-assisted barley breeding. The new maps are available on-line at www.genica.net.au.

Details

Title: New eSSR and gSSR markers added to Australian barley maps
Authors/Creators: K.L. Willsmore (Author/Creator) - South Australian Research and Development Institute
P. Eckermann (Author/Creator) - The University of Adelaide
R.K. Varshney (Author/Creator) - Australian Centre for Plant Functional Genomics
A. Graner (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
P. Langridge (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
M. Pallotta (Author/Creator) - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
J. Cheong (Author/Creator) - South Australian Research and Development Institute
Kevin J. Williams (Author/Creator)
Publication Details: Australian Journal of Agricultural Research, Vol.57(9), pp.953-959
Publisher: CSIRO
Identifiers: 991005546040307891
Copyright: © 2006 CSIRO
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agriculture, Multidisciplinary
ESI research areas: Agricultural Sciences