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Journal article
New fungal primers reveal the diversity of Mucoromycotinian arbuscular mycorrhizal fungi and their response to nitrogen application
Environmental microbiome, Vol.19(1), 71
2024
PMID: 39294800
Abstract
Background
Arbuscular mycorrhizas (AM) are the most widespread terrestrial symbiosis and are both a key determinant of plant health and a major contributor to ecosystem processes through their role in biogeochemical cycling. Until recently, it was assumed that the fungi which form AM comprise the subphylum Glomeromycotina (G-AMF), and our understanding of the diversity and ecosystem roles of AM is based almost exclusively on this group. However recent evidence shows that fungi which form the distinctive 'fine root endophyte’ (FRE) AM morphotype are members of the subphylum Mucoromycotina (M-AMF), so that AM symbioses are actually formed by two distinct groups of fungi.
Results
We investigated the influence of nitrogen (N) addition and wheat variety on the assembly of AM communities under field conditions. Visual assessment of roots showed co-occurrence of G-AMF and M-AMF, providing an opportunity to compare the responses of these two groups. Existing ‘AM’ 18S rRNA primers which co-amplify G-AMF and M-AMF were modified to reduce bias against Mucoromycotina, and compared against a new ‘FRE’ primer set which selectively amplifies Mucoromycotina. Using the AM-primers, no significant effect of either N-addition or wheat variety on G-AMF or M-AMF diversity or community composition was detected. In contrast, using the FRE-primers, N-addition was shown to reduce M-AMF diversity and altered community composition. The ASV which responded to N-addition were closely related, demonstrating a clear phylogenetic signal which was identified only by the new FRE-primers. The most abundant Mucoromycotina sequences we detected belonged to the same Endogonales clades as dominant sequences associated with FRE morphology in Australia, indicating that closely related M-AMF may be globally distributed.
Conclusions
The results demonstrate the need to consider both G-AMF and M-AMF when investigating AM communities, and highlight the importance of primer choice when investigating AMF community dynamics.
Details
- Title
- New fungal primers reveal the diversity of Mucoromycotinian arbuscular mycorrhizal fungi and their response to nitrogen application
- Authors/Creators
- Mirjam Seeliger - Newcastle upon Tyne, NE1 7RU UKSally Hilton - CoventryGeorge Muscatt - CoventryChristopher Walker - 21A Inverleith Row, Edinburgh, EH3 5LR UK Crawley, WA 6009 AustraliaDavid Bass - Barrack Road, The Nothe, Weymouth, DT4 8UB UK Cromwell Road, London, SW7 5BD UKFelipe Albornoz - Murdoch University, Centre for Terrestrial Ecosystem Science and SustainabilityRachel J. Standish - Murdoch University, School of Environmental and Conservation SciencesNeil D. Gray - Newcastle upon Tyne, NE1 7RU UKLouis Mercy - INOQ Gmbh, 29465 Schnega, GermanyLeonidas Rempelos - Newcastle upon Tyne, NE1 7RU UKCarolin Schneider - INOQ Gmbh, 29465 Schnega, GermanyMegan H. Ryan - The University of Western AustraliaPaul E. Bilsborrow - Newcastle upon Tyne, NE1 7RU UKGary D. Bending - Coventry
- Publication Details
- Environmental microbiome, Vol.19(1), 71
- Publisher
- BioMed Central
- Identifiers
- 991005703768707891
- Copyright
- © The Author(s) 2024.
- Murdoch Affiliation
- School of Environmental and Conservation Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.488 Mycorrhizal Symbiosis
- Web Of Science research areas
- Genetics & Heredity
- Microbiology
- ESI research areas
- Molecular Biology & Genetics